8hps: Difference between revisions

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-'''Unreleased structure'''
-The entry 8hps is ON HOLD
+==LpqY-SugABC in state 5==
+<StructureSection load='8hps' size='340' side='right'caption='[[8hps]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8hps]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HPS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.51&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hps OCA], [https://pdbe.org/8hps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hps RCSB], [https://www.ebi.ac.uk/pdbsum/8hps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hps ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/I7G6S2_MYCS2 I7G6S2_MYCS2]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The human pathogen, Mycobacterium tuberculosis (Mtb) relies heavily on trehalose for both survival and pathogenicity. The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the only trehalose import pathway in Mtb. Conformational dynamics of ABC transporters is an important feature to explain how they operate, but experimental structures are determined in a static environment. Therefore, a detailed transport mechanism cannot be elucidated because there is a lack of intermediate structures. Here, we used single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the Mycobacterium smegmatis (M. smegmatis) trehalose-specific importer LpqY-SugABC complex in five different conformations. These structures have been classified and reconstructed from a single cryo-EM dataset. This study allows a comprehensive understanding of the trehalose recycling mechanism in Mycobacteria and also demonstrates the potential of single-particle cryo-EM to explore the dynamic structures of other ABC transporters and molecular machines.
-Authors: Liang, J., Yang, X., Zhang, B., Rao, Z., Liu, F.
+Structural insights into trehalose capture and translocation by mycobacterial LpqY-SugABC.,Liang J, Yang X, Hu T, Gao Y, Yang Q, Yang H, Peng W, Zhou X, Guddat LW, Zhang B, Rao Z, Liu F Structure. 2023 Aug 10:S0969-2126(23)00276-9. doi: 10.1016/j.str.2023.07.014. PMID:37619560<ref>PMID:37619560</ref>
-Description: LpqY-SugABC in catalytic intermediate state
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Zhang, B]]
+<div class="pdbe-citations 8hps" style="background-color:#fffaf0;"></div>
-[[Category: Yang, X]]
+== References ==
-[[Category: Liu, F]]
+<references/>
-[[Category: Liang, J]]
+__TOC__
-[[Category: Rao, Z]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycolicibacterium smegmatis MC2 155]]
+[[Category: Liang J]]
+[[Category: Liu F]]
+[[Category: Rao Z]]
+[[Category: Yang X]]
+[[Category: Zhang B]]