5ik4: Difference between revisions

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-'''Unreleased structure'''
-The entry 5ik4 is ON HOLD
+==Laminin A2LG45 C-form, Apo.==
+<StructureSection load='5ik4' size='340' side='right'caption='[[5ik4]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ik4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IK4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ik4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ik4 OCA], [https://pdbe.org/5ik4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ik4 RCSB], [https://www.ebi.ac.uk/pdbsum/5ik4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ik4 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE] Note=Defects in Lama2 are a cause of murine muscular dystrophy (dy2J).
+== Function ==
+[https://www.uniprot.org/uniprot/LAMA2_MOUSE LAMA2_MOUSE] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dystroglycan is a highly glycosylated extracellular matrix receptor with essential functions in skeletal muscle and the nervous system. Reduced matrix binding by alpha-dystroglycan (alpha-DG) due to perturbed glycosylation is a pathological feature of several forms of muscular dystrophy. Like-acetylglucosaminyltransferase (LARGE) synthesizes the matrix-binding heteropolysaccharide [-glucuronic acid-beta1,3-xylose-alpha1,3-]n. Using a dual exoglycosidase digestion, we confirm that this polysaccharide is present on native alpha-DG from skeletal muscle. The atomic details of matrix binding were revealed by a high-resolution crystal structure of laminin-G-like (LG) domains 4 and 5 (LG4 and LG5) of laminin-alpha2 bound to a LARGE-synthesized oligosaccharide. A single glucuronic acid-beta1,3-xylose disaccharide repeat straddles a Ca2+ ion in the LG4 domain, with oxygen atoms from both sugars replacing Ca2+-bound water molecules. The chelating binding mode accounts for the high affinity of this protein-carbohydrate interaction. These results reveal a previously uncharacterized mechanism of carbohydrate recognition and provide a structural framework for elucidating the mechanisms underlying muscular dystrophy.
-Authors: Briggs, D.C., Hohenester, E., Campbell, K.P.
+Structural basis of laminin binding to the LARGE glycans on dystroglycan.,Briggs DC, Yoshida-Moriguchi T, Zheng T, Venzke D, Anderson ME, Strazzulli A, Moracci M, Yu L, Hohenester E, Campbell KP Nat Chem Biol. 2016 Aug 15. doi: 10.1038/nchembio.2146. PMID:27526028<ref>PMID:27526028</ref>
-Description: Laminin A2LG45 C-form, Apo.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Hohenester, E]]
+<div class="pdbe-citations 5ik4" style="background-color:#fffaf0;"></div>
-[[Category: Campbell, K.P]]
-[[Category: Briggs, D.C]]
+==See Also==
+*[[Laminin|Laminin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Briggs DC]]
+[[Category: Campbell KP]]
+[[Category: Hohenester E]]