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==Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature==
==Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature==
<StructureSection load='5igd' size='340' side='right' caption='[[5igd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='5igd' size='340' side='right'caption='[[5igd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5igd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IGD FirstGlance]. <br>
<table><tr><td colspan='2'>[[5igd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IGD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THP:THYMIDINE-3,5-DIPHOSPHATE'>THP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bdc|3bdc]], [[5i9p|5i9p]], [[1tqo|1tqo]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THP:THYMIDINE-3,5-DIPHOSPHATE'>THP</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nuc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5igd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igd OCA], [https://pdbe.org/5igd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5igd RCSB], [https://www.ebi.ac.uk/pdbsum/5igd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5igd ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5igd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5igd OCA], [http://pdbe.org/5igd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5igd RCSB], [http://www.ebi.ac.uk/pdbsum/5igd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5igd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU]] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.  
[https://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.
 
==See Also==
*[[Staphylococcal nuclease 3D structures|Staphylococcal nuclease 3D structures]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Micrococcal nuclease]]
[[Category: Large Structures]]
[[Category: E., B Garcia-Moreno]]
[[Category: Staphylococcus aureus]]
[[Category: Robinson, A C]]
[[Category: Garcia-Moreno E B]]
[[Category: Schlessman, J L]]
[[Category: Robinson AC]]
[[Category: Theodoru, A]]
[[Category: Schlessman JL]]
[[Category: Hydrolase]]
[[Category: Theodoru A]]
[[Category: Hyperstable]]
[[Category: Ionizable group]]
[[Category: Nuclease]]
[[Category: Pdtp]]

Latest revision as of 16:51, 30 August 2023

Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperatureCrystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature

Structural highlights

5igd is a 1 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUC_STAAU Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.

See Also

5igd, resolution 1.80Å

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