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[[Image:2bn5.gif|left|200px]]
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{{STRUCTURE_2bn5|  PDB=2bn5  |  SCENE=  }}
'''P-ELEMENT SOMATIC INHIBITOR PROTEIN COMPLEX WITH U1-70K PROLINE-RICH PEPTIDE'''


==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide==
<StructureSection load='2bn5' size='340' side='right'caption='[[2bn5]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BN5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn5 OCA], [https://pdbe.org/2bn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bn5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7JPS0_DROSP Q7JPS0_DROSP]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.


==Overview==
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112<ref>PMID:15990112</ref>
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2BN5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA].
</div>
<div class="pdbe-citations 2bn5" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15990112 15990112]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Butler, P J.G.]]
[[Category: Butler PJG]]
[[Category: Ignjatovic, T.]]
[[Category: Ignjatovic T]]
[[Category: Nagai, K.]]
[[Category: Nagai K]]
[[Category: Neuhaus, D.]]
[[Category: Neuhaus D]]
[[Category: Yang, J C.]]
[[Category: Yang J-C]]
[[Category: Complex]]
[[Category: Inhibitor]]
[[Category: Nmr structure]]
[[Category: Nuclear protein/complex]]
[[Category: Proline-rich peptide]]
[[Category: Protein-protein interaction]]
[[Category: Splicing]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:31:20 2008''

Latest revision as of 16:35, 30 August 2023

P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptideP-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide

Structural highlights

2bn5 is a 2 chain structure with sequence from Drosophila melanogaster. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7JPS0_DROSP

Publication Abstract from PubMed

P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.

Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K. Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase. J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112 doi:S0022-2836(05)00543-7
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