3cx8: Difference between revisions

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-{{Seed}}
-[[Image:3cx8.png|left|200px]]
-<!--
+==Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GTP-gamma-S==
-The line below this paragraph, containing "STRUCTURE_3cx8", creates the "Structure Box" on the page.
+<StructureSection load='3cx8' size='340' side='right'caption='[[3cx8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3cx8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CX8 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-{{STRUCTURE_3cx8|  PDB=3cx8  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cx8 OCA], [https://pdbe.org/3cx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cx8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cx8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GNA13_MOUSE GNA13_MOUSE] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/3cx8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cx8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+G12 class heterotrimeric G proteins stimulate RhoA activation by RGS-RhoGEFs. However, p115RhoGEF is a GTPase Activating Protein (GAP) toward Galpha13, whereas PDZRhoGEF is not. We have characterized the interaction between the PDZRhoGEF rgRGS domain (PRG-rgRGS) and the alpha subunit of G13 and have determined crystal structures of their complexes in both the inactive state bound to GDP and the active states bound to GDP*AlF (transition state) and GTPgammaS (Michaelis complex). PRG-rgRGS interacts extensively with the helical domain and the effector-binding sites on Galpha13 through contacts that are largely conserved in all three nucleotide-bound states, although PRG-rgRGS has highest affinity to the Michaelis complex. An acidic motif in the N terminus of PRG-rgRGS occupies the GAP binding site of Galpha13 and is flexible in the GDP*AlF complex but well ordered in the GTPgammaS complex. Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity.
-===Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GTP-gamma-S===
+Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF.,Chen Z, Singer WD, Danesh SM, Sternweis PC, Sprang SR Structure. 2008 Oct 8;16(10):1532-43. PMID:18940608<ref>PMID:18940608</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18940608}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3cx8" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18940608 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18940608}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-CX8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CX8 OCA].
-==Reference==
-<ref group="xtra">PMID:18940608</ref><references group="xtra"/>
 [[Category: Mus musculus]]
 [[Category: Rattus norvegicus]]
-[[Category: Chen, Z.]]
+[[Category: Chen Z]]
-[[Category: Sprang, S R.]]
+[[Category: Sprang SR]]
-[[Category: Coiled coil]]
-[[Category: Cytoplasm]]
-[[Category: Gtp-binding]]
-[[Category: Gtpase activation]]
-[[Category: Guanine-nucleotide releasing factor]]
-[[Category: Lipoprotein]]
-[[Category: Membrane]]
-[[Category: Nucleotide-binding]]
-[[Category: Palmitate]]
-[[Category: Phosphoprotein]]
-[[Category: Protein complex]]
-[[Category: Signal transduction]]
-[[Category: Signaling protein]]
-[[Category: Transducer]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:36:45 2009''