3cpr: Difference between revisions

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-[[Image:3cpr.png|left|200px]]
-{{STRUCTURE_3cpr|  PDB=3cpr  |  SCENE=  }}
+==The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution==
+<StructureSection load='3cpr' size='340' side='right'caption='[[3cpr]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3cpr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPR FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KPI:(2S)-2-AMINO-6-[(1-HYDROXY-1-OXO-PROPAN-2-YLIDENE)AMINO]HEXANOIC+ACID'>KPI</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpr OCA], [https://pdbe.org/3cpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpr RCSB], [https://www.ebi.ac.uk/pdbsum/3cpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpr ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DAPA_CORGL DAPA_CORGL] Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).[HAMAP-Rule:MF_00418]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpr_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cpr ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The lysine insensitive Corynebacterium glutamicum dihydrodipicolinate synthase enzyme (cDHDPS) was recently successfully introduced into maize plants to enhance the level of lysine in the grain. To better understand lysine insensitivity of the cDHDPS, we expressed, purified, kinetically characterized the protein, and solved its X-ray crystal structure. The cDHDPS enzyme has a fold and overall structure that is highly similar to other DHDPS proteins. A noteworthy feature of the active site is the evidence that the catalytic lysine residue forms a Schiff base adduct with pyruvate. Analyses of the cDHDPS structure in the vicinity of the putative binding site for S-lysine revealed that the allosteric binding site in the Escherichia coli DHDPS protein does not exist in cDHDPS due to three non-conservative amino acids substitutions, and this is likely why cDHDPS is not feedback inhibited by lysine.
-===The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution===
+Characterization and crystal structure of lysine insensitive Corynebacterium glutamicum dihydrodipicolinate synthase (cDHDPS) protein.,Rice EA, Bannon GA, Glenn KC, Jeong SS, Sturman EJ, Rydel TJ Arch Biochem Biophys. 2008 Dec 15;480(2):111-21. Epub 2008 Oct 7. PMID:18930704<ref>PMID:18930704</ref>
-{{ABSTRACT_PUBMED_18930704}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3cpr" style="background-color:#fffaf0;"></div>
-[[3cpr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPR OCA].
 ==See Also==
-*[[Dihydrodipicolinate Synthase|Dihydrodipicolinate Synthase]]
+*[[Dihydrodipicolinate synthase|Dihydrodipicolinate synthase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Corynebacterium glutamicum]]
-[[Category: Dihydrodipicolinate synthase]]
+[[Category: Large Structures]]
-[[Category: Rice, E A.]]
+[[Category: Rice EA]]
-[[Category: Rydel, T J.]]
+[[Category: Rydel TJ]]
-[[Category: Sturman, E J.]]
+[[Category: Sturman EJ]]
-[[Category: Amino-acid biosynthesis]]
-[[Category: Diaminopimelate biosynthesis]]
-[[Category: Lyase]]
-[[Category: Lysine biosynthesis]]
-[[Category: Schiff base]]