3bue: Difference between revisions

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[[Image:3bue.jpg|left|200px]]


{{Structure
==Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis==
|PDB= 3bue |SIZE=350|CAPTION= <scene name='initialview01'>3bue</scene>, resolution 2.15&Aring;
<StructureSection load='3bue' size='340' side='right'caption='[[3bue]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[3bue]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BUE FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
|GENE= argR, ahrC, Rv1657, MT1695, MTCY06H11.22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bue OCA], [https://pdbe.org/3bue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bue RCSB], [https://www.ebi.ac.uk/pdbsum/3bue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bue ProSAT], [https://www.topsan.org/Proteins/TBSGC/3bue TOPSAN]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARGR_MYCTU ARGR_MYCTU] Regulates arginine biosynthesis genes (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/3bue_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bue ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Mycobacterium tuberculosis (Mtb) gene product encoded by open reading frame Rv1657 is an arginine repressor (ArgR). All genes involved in the L-arginine (hereafter arginine) biosynthetic pathway are essential for optimal growth of the Mtb pathogen, thus making MtbArgR a potential target for drug design. The C-terminal domains of arginine repressors (CArgR) participate in oligomerization and arginine binding. Several crystal forms of CArgR from Mtb (MtbCArgR) have been obtained. The X-ray crystal structures of MtbCArgR were determined at 1.85 A resolution with bound arginine and at 2.15 A resolution in the unliganded form. These structures show that six molecules of MtbCArgR are arranged into a hexamer having approximate 32 point symmetry that is formed from two trimers. The trimers rotate relative to each other by about 11 degrees upon binding arginine. All residues in MtbCArgR deemed to be important for hexamer formation and for arginine binding have been identified from the experimentally determined structures presented. The hexamer contains six regular sites in which the arginine molecules have one common binding mode and three sites in which the arginine molecules have two overlapping binding modes. The latter sites only bind the ligand at high (200 mM) arginine concentrations.


'''Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis'''
Structure of the C-terminal domain of the arginine repressor protein from Mycobacterium tuberculosis.,Cherney LT, Cherney MM, Garen CR, Lu GJ, James MN Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):950-6. Epub 2008, Aug 13. PMID:18703843<ref>PMID:18703843</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3bue" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The gene product of an open reading frame Rv1657 from Mycobacterium tuberculosis is a putative arginine repressor protein (ArgR), a transcriptional factor that regulates the expression of arginine-biosynthetic enzymes. Rv1657 was expressed and purified and a C-terminal domain was crystallized using the hanging-drop vapour-diffusion method. Diffraction data were collected and processed to a resolution of 2.15 A. The crystals belong to space group P1 and the Matthews coefficient suggests that the crystals contain six C-terminal domain molecules per unit cell. Previous structural and biochemical studies on the arginine repressor proteins from other organisms have likewise shown the presence of six molecules per unit cell.
*[[Arginine repressor 3D structures|Arginine repressor 3D structures]]
 
== References ==
==About this Structure==
<references/>
3BUE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BUE OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Expression, purification and preliminary X-ray analysis of the C-terminal domain of an arginine repressor protein from Mycobacterium tuberculosis., Lu GJ, Garen CR, Cherney MM, Cherney LT, Lee C, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt, 11):936-9. Epub 2007 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18007044 18007044]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Mycobacterium tuberculosis h37rv]]
[[Category: Cherney LT]]
[[Category: Single protein]]
[[Category: Cherney MM]]
[[Category: Cherney, L T.]]
[[Category: Garen CR]]
[[Category: Cherney, M M.]]
[[Category: James MNG]]
[[Category: Garen, C R.]]
[[Category: Lu GJ]]
[[Category: James, M N.G.]]
[[Category: Lu, G J.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: amino-acid biosynthesis]]
[[Category: arginine biosynthesis]]
[[Category: cytoplasm]]
[[Category: dna binding protein]]
[[Category: dna-binding]]
[[Category: hexamer]]
[[Category: l-arginine binding domain]]
[[Category: l-arginine repressor protein]]
[[Category: oligomerization domain]]
[[Category: repressor]]
[[Category: structural genomic]]
[[Category: tb structural genomics consortium]]
[[Category: tbsgc]]
[[Category: transcription]]
[[Category: transcription regulation]]
 
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