3bue: Difference between revisions

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-[[Image:3bue.png|left|200px]]
-{{STRUCTURE_3bue|  PDB=3bue  |  SCENE=  }}
+==Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis==
+<StructureSection load='3bue' size='340' side='right'caption='[[3bue]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3bue]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BUE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bue OCA], [https://pdbe.org/3bue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bue RCSB], [https://www.ebi.ac.uk/pdbsum/3bue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bue ProSAT], [https://www.topsan.org/Proteins/TBSGC/3bue TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARGR_MYCTU ARGR_MYCTU] Regulates arginine biosynthesis genes (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/3bue_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bue ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Mycobacterium tuberculosis (Mtb) gene product encoded by open reading frame Rv1657 is an arginine repressor (ArgR). All genes involved in the L-arginine (hereafter arginine) biosynthetic pathway are essential for optimal growth of the Mtb pathogen, thus making MtbArgR a potential target for drug design. The C-terminal domains of arginine repressors (CArgR) participate in oligomerization and arginine binding. Several crystal forms of CArgR from Mtb (MtbCArgR) have been obtained. The X-ray crystal structures of MtbCArgR were determined at 1.85 A resolution with bound arginine and at 2.15 A resolution in the unliganded form. These structures show that six molecules of MtbCArgR are arranged into a hexamer having approximate 32 point symmetry that is formed from two trimers. The trimers rotate relative to each other by about 11 degrees upon binding arginine. All residues in MtbCArgR deemed to be important for hexamer formation and for arginine binding have been identified from the experimentally determined structures presented. The hexamer contains six regular sites in which the arginine molecules have one common binding mode and three sites in which the arginine molecules have two overlapping binding modes. The latter sites only bind the ligand at high (200 mM) arginine concentrations.
-===Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis===
+Structure of the C-terminal domain of the arginine repressor protein from Mycobacterium tuberculosis.,Cherney LT, Cherney MM, Garen CR, Lu GJ, James MN Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):950-6. Epub 2008, Aug 13. PMID:18703843<ref>PMID:18703843</ref>
-{{ABSTRACT_PUBMED_18703843}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3bue" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3bue]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BUE OCA].
+*[[Arginine repressor 3D structures|Arginine repressor 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:018703843</ref><ref group="xtra">PMID:018007044</ref><references group="xtra"/>
+__TOC__
-[[Category: Mycobacterium tuberculosis]]
+</StructureSection>
-[[Category: Cherney, L T.]]
+[[Category: Large Structures]]
-[[Category: Cherney, M M.]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-[[Category: Garen, C R.]]
+[[Category: Cherney LT]]
-[[Category: James, M N.G.]]
+[[Category: Cherney MM]]
-[[Category: Lu, G J.]]
+[[Category: Garen CR]]
-[[Category: TBSGC, TB Structural Genomics Consortium.]]
+[[Category: James MNG]]
-[[Category: Amino-acid biosynthesis]]
+[[Category: Lu GJ]]
-[[Category: Arginine biosynthesis]]
-[[Category: Dna binding protein]]
-[[Category: Dna-binding]]
-[[Category: Hexamer]]
-[[Category: L-arginine binding domain]]
-[[Category: L-arginine repressor protein]]
-[[Category: Oligomerization domain]]
-[[Category: Repressor]]
-[[Category: Structural genomic]]
-[[Category: Tb structural genomics consortium]]
-[[Category: Tbsgc]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]