3bgv: Difference between revisions

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[[Image:3bgv.jpg|left|200px]]


{{Structure
==Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with SAH==
|PDB= 3bgv |SIZE=350|CAPTION= <scene name='initialview01'>3bgv</scene>, resolution 2.30&Aring;
<StructureSection load='3bgv' size='340' side='right'caption='[[3bgv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
<table><tr><td colspan='2'>[[3bgv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BGV FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
|GENE= RNMT, KIAA0398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bgv OCA], [https://pdbe.org/3bgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bgv RCSB], [https://www.ebi.ac.uk/pdbsum/3bgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bgv ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bgv OCA], [http://www.ebi.ac.uk/pdbsum/3bgv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bgv RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/MCES_HUMAN MCES_HUMAN] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:9790902</ref> <ref>PMID:9705270</ref> <ref>PMID:10347220</ref> <ref>PMID:22099306</ref>
 
== Evolutionary Conservation ==
'''The crystals structure of mRNA cap guanine-N7 methyltransferase'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/3bgv_consurf.spt"</scriptWhenChecked>
3BGV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGV OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bgv ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: mRNA (guanine-N(7)-)-methyltransferase]]
[[Category: Antoshenko T]]
[[Category: Antoshenko, T.]]
[[Category: Arrowsmith CH]]
[[Category: Arrowsmith, C H.]]
[[Category: Bochkarev A]]
[[Category: Bochkarev, A.]]
[[Category: Edwards AM]]
[[Category: Edwards, A M.]]
[[Category: Lunin VV]]
[[Category: Lunin, V V.]]
[[Category: MacKenzie F]]
[[Category: MacKenzie, F.]]
[[Category: Min J]]
[[Category: Min, J.]]
[[Category: Plotnikov AN]]
[[Category: Plotnikov, A N.]]
[[Category: Weigelt J]]
[[Category: SGC, Structural Genomics Consortium.]]
[[Category: Wu H]]
[[Category: Weigelt, J.]]
[[Category: Zeng H]]
[[Category: Wu, H.]]
[[Category: Zeng, H.]]
[[Category: alternative splicing]]
[[Category: methyltransferase]]
[[Category: mrna capping]]
[[Category: mrna processing]]
[[Category: nucleus]]
[[Category: phosphoprotein]]
[[Category: rna-binding]]
[[Category: s-adenosyl-l-methionine]]
[[Category: sgc]]
[[Category: structural genomic]]
[[Category: structural genomics consortium]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:26:09 2008''

Latest revision as of 15:08, 30 August 2023

Crystal structure of mRNA cap guanine-N7 methyltransferase in complex with SAHCrystal structure of mRNA cap guanine-N7 methyltransferase in complex with SAH

Structural highlights

3bgv is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MCES_HUMAN mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Tsukamoto T, Shibagaki Y, Niikura Y, Mizumoto K. Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase. Biochem Biophys Res Commun. 1998 Oct 9;251(1):27-34. PMID:9790902 doi:http://dx.doi.org/S0006-291X(98)99402-5
  2. ↑ Pillutla RC, Yue Z, Maldonado E, Shatkin AJ. Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. J Biol Chem. 1998 Aug 21;273(34):21443-6. PMID:9705270
  3. ↑ Saha N, Schwer B, Shuman S. Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. J Biol Chem. 1999 Jun 4;274(23):16553-62. PMID:10347220
  4. ↑ Gonatopoulos-Pournatzis T, Dunn S, Bounds R, Cowling VH. RAM/Fam103a1 is required for mRNA cap methylation. Mol Cell. 2011 Nov 18;44(4):585-96. doi: 10.1016/j.molcel.2011.08.041. PMID:22099306 doi:http://dx.doi.org/10.1016/j.molcel.2011.08.041

3bgv, resolution 2.30Å

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