2zj0: Difference between revisions

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-{{Seed}}
-[[Image:2zj0.png|left|200px]]
-<!--
+==Crystal structure of Mycobacterium tuberculosis S-Adenosyl-L-homocysteine hydrolase in ternary complex with NAD and 2-fluoroadenosine==
-The line below this paragraph, containing "STRUCTURE_2zj0", creates the "Structure Box" on the page.
+<StructureSection load='2zj0' size='340' side='right'caption='[[2zj0]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2zj0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZJ0 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2FA:2-(6-AMINO-2-FLUORO-PURIN-9-YL)-5-HYDROXYMETHYL-TETRAHYDRO-FURAN-3,4-DIOL'>2FA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-{{STRUCTURE_2zj0|  PDB=2zj0  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zj0 OCA], [https://pdbe.org/2zj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zj0 RCSB], [https://www.ebi.ac.uk/pdbsum/2zj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zj0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SAHH_MYCTU SAHH_MYCTU] May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine (By similarity).[HAMAP-Rule:MF_00563]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/2zj0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zj0 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+S-adenosylhomocysteine hydrolase (SAHH) is a ubiquitous enzyme that plays a central role in methylation-based processes by maintaining the intracellular balance between S-adenosylhomocysteine (SAH) and S-adenosylmethionine. We report the first prokaryotic crystal structure of SAHH, from Mycobacterium tuberculosis (Mtb), in complex with adenosine (ADO) and nicotinamide adenine dinucleotide. Structures of complexes with three inhibitors are also reported: 3'-keto aristeromycin (ARI), 2-fluoroadenosine, and 3-deazaadenosine. The ARI complex is the first reported structure of SAHH complexed with this inhibitor, and confirms the oxidation of the 3' hydroxyl to a planar keto group, consistent with its prediction as a mechanism-based inhibitor. We demonstrate the in vivo enzyme inhibition activity of the three inhibitors and also show that 2-fluoradenosine has bactericidal activity. While most of the residues lining the ADO-binding pocket are identical between Mtb and human SAHH, less is known about the binding mode of the homocysteine (HCY) appendage of the full substrate. We report the 2.0 A resolution structure of the complex of SAHH cocrystallized with SAH. The most striking change in the structure is that binding of HCY forces a rotation of His363 around the backbone to flip out of contact with the 5' hydroxyl of the ADO and opens access to a nearby channel that leads to the surface. This complex suggests that His363 acts as a switch that opens up to permit binding of substrate, then closes down after release of the cleaved HCY. Differences in the entrance to this access channel between human and Mtb SAHH are identified.
-===Crystal structure of Mycobacterium tuberculosis S-Adenosyl-L-homocysteine hydrolase in ternary complex with NAD and 2-fluoroadenosine===
+Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors.,Reddy MC, Kuppan G, Shetty ND, Owen JL, Ioerger TR, Sacchettini JC Protein Sci. 2008 Dec;17(12):2134-44. Epub 2008 Sep 24. PMID:18815415<ref>PMID:18815415</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2zj0" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18815415}}, adds the Publication Abstract to the page
+*[[S-adenosylhomocysteine hydrolase|S-adenosylhomocysteine hydrolase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18815415 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18815415}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-ZJ0 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJ0 OCA].
+[[Category: Mycobacterium tuberculosis H37Rv]]
+[[Category: Gokulan K]]
-==Reference==
+[[Category: Ioerger TR]]
-Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors., Reddy MC, Kuppan G, Shetty ND, Owen JL, Ioerger TR, Sacchettini JC, Protein Sci. 2008 Dec;17(12):2134-44. Epub 2008 Sep 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18815415 18815415]
+[[Category: Owen JL]]
-[[Category: Adenosylhomocysteinase]]
+[[Category: Reddy MCM]]
-[[Category: Mycobacterium tuberculosis h37rv]]
+[[Category: Sacchettini JC]]
-[[Category: Gokulan, K.]]
+[[Category: Shetty ND]]
-[[Category: Ioerger, T R.]]
-[[Category: Owen, J L.]]
-[[Category: Reddy, M C.M.]]
-[[Category: Sacchettini, J C.]]
-[[Category: Shetty, N D.]]
-[[Category: 37 amino acid insertional region]]
-[[Category: Cytoplasm]]
-[[Category: Dimer of dimer]]
-[[Category: Hydrolase]]
-[[Category: Nad binding domain]]
-[[Category: One-carbon metabolism]]
-[[Category: Protein-substrate complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 15:43:37 2008''