2qpl: Difference between revisions

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-[[Image:2qpl.jpg|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_2qpl", creates the "Structure Box" on the page.
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-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-{{STRUCTURE_2qpl|  PDB=2qpl  |  SCENE=  }}
-'''Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue'''
+==Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue==
+<StructureSection load='2qpl' size='340' side='right'caption='[[2qpl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2qpl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QPL FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTY:4-AMINO-7-METHYLPYRAZOLO[1,5-A][1,3,5]TRIAZIN-2(1H)-ONE'>BTY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qpl OCA], [https://pdbe.org/2qpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qpl RCSB], [https://www.ebi.ac.uk/pdbsum/2qpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qpl ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PNPH_BOVIN PNPH_BOVIN] The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/2qpl_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qpl ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The combined use of a rapid virtual screen of a small fragment library together with a single point enzyme assay has been used for the discovery of novel PNP inhibitors. The availability of readily soakable crystals of bovine PNP has allowed the approach to be experimentally validated by determining the crystal structure of one of the inhibitor-PNP complexes. Comparison of the experimentally determined binding mode with that predicted by the virtual screening shows them to be similar. This represents a starting point for the growth of the ligand into a higher affinity inhibitor.
-==Overview==
+Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue.,Pereira HM, Berdini V, Cleasby A, Garratt RC FEBS Lett. 2007 Oct 30;581(26):5082-6. Epub 2007 Oct 2. PMID:17927987<ref>PMID:17927987</ref>
-The combined use of a rapid virtual screen of a small fragment library together with a single point enzyme assay has been used for the discovery of novel PNP inhibitors. The availability of readily soakable crystals of bovine PNP has allowed the approach to be experimentally validated by determining the crystal structure of one of the inhibitor-PNP complexes. Comparison of the experimentally determined binding mode with that predicted by the virtual screening shows them to be similar. This represents a starting point for the growth of the ligand into a higher affinity inhibitor.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-QPL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QPL OCA].
+</div>
+<div class="pdbe-citations 2qpl" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue., Pereira HM, Berdini V, Cleasby A, Garratt RC, FEBS Lett. 2007 Oct 30;581(26):5082-6. Epub 2007 Oct 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17927987 17927987]
+*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
-[[Category: Purine-nucleoside phosphorylase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Berdini V]]
-[[Category: Berdini, V.]]
+[[Category: Cleasby A]]
-[[Category: Cleasby, A.]]
+[[Category: Garratt RC]]
-[[Category: Garratt, R C.]]
+[[Category: Pereira HM]]
-[[Category: Pereira, H M.]]
-[[Category: Acetylation]]
-[[Category: Glycosyltransferase]]
-[[Category: Purine nucleoside phosphorylase]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 15:22:26 2008''