2p18: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2p18.png|left|200px]]
-<!--
+==Crystal structure of the Leishmania infantum glyoxalase II==
-The line below this paragraph, containing "STRUCTURE_2p18", creates the "Structure Box" on the page.
+<StructureSection load='2p18' size='340' side='right'caption='[[2p18]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2p18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P18 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_2p18|  PDB=2p18  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p18 OCA], [https://pdbe.org/2p18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p18 RCSB], [https://www.ebi.ac.uk/pdbsum/2p18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p18 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2PYN0_LEIIN Q2PYN0_LEIIN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p1/2p18_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p18 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The glyoxalase pathway catalyzes the formation of d-lactate from methylglyoxal, a toxic byproduct of glycolysis. In trypanosomatids, trypanothione replaces glutathione in this pathway, making it a potential drug target, since its selective inhibition might increase methylglyoxal concentration in the parasites. Two glyoxalase II structures were solved. One with a bound spermidine molecule (1.8 A) and the other with d-lactate at the active site (1.9 A). The second structure was obtained by crystal soaking with the enzyme substrate (S)-d-lactoyltrypanothione. The overall structure of Leishmania infantum glyoxalase II is very similar to its human counterpart, with important differences at the substrate binding site. The crystal structure of L. infantum glyoxalase II is the first structure of this enzyme from trypanosomatids. The differential specificity of glyoxalase II toward glutathione and trypanothione moieties was revealed by differential substrate binding. Evolutionary analysis shows that trypanosomatid glyoxalases II diverged early from eukaryotic enzymes, being unrelated to prokaryotic proteins.
-===Crystal structure of the Leishmania infantum glyoxalase II===
+Catalysis and structural properties of Leishmania infantum glyoxalase II: trypanothione specificity and phylogeny.,Silva MS, Barata L, Ferreira AE, Romao S, Tomas AM, Freire AP, Cordeiro C Biochemistry. 2008 Jan 8;47(1):195-204. Epub 2007 Dec 6. PMID:18052346<ref>PMID:18052346</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2p18" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18052346}}, adds the Publication Abstract to the page
+*[[Glyoxalase 3D structures|Glyoxalase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18052346 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18052346}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2p18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P18 OCA].
-==Reference==
-<ref group="xtra">PMID:018052346</ref><references group="xtra"/>
-[[Category: Hydroxyacylglutathione hydrolase]]
 [[Category: Leishmania infantum]]
-[[Category: Barata, L.]]
+[[Category: Barata L]]
-[[Category: Bonifacio, C.]]
+[[Category: Bonifacio C]]
-[[Category: Najmudin, S.]]
+[[Category: Najmudin S]]
-[[Category: Romao, M J.]]
+[[Category: Romao MJ]]
-[[Category: Trincao, J.]]
+[[Category: Trincao J]]
-[[Category: Alpha-helical domain]]
-[[Category: Beta sandwich]]
-[[Category: Hydrolase]]
-[[Category: Metalloprotein]]