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[[Image:2os8.jpg|left|200px]]
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{{STRUCTURE_2os8|  PDB=2os8  |  SCENE=  }}
'''Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor'''


==Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor==
<StructureSection load='2os8' size='340' side='right'caption='[[2os8]], [[Resolution|resolution]] 3.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2os8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Placopecten_magellanicus Placopecten magellanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OS8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2os8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2os8 OCA], [https://pdbe.org/2os8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2os8 RCSB], [https://www.ebi.ac.uk/pdbsum/2os8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2os8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q26080_PLAMG Q26080_PLAMG]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/2os8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2os8 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states.


==Overview==
Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor.,Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C Structure. 2007 May;15(5):553-64. PMID:17502101<ref>PMID:17502101</ref>
Unlike processive cellular motors such as myosin V, whose structure has recently been determined in a "rigor-like" conformation, myosin II from contracting muscle filaments necessarily spends most of its time detached from actin. By using squid and sea scallop sources, however, we have now obtained similar rigor-like atomic structures for muscle myosin heads (S1). The significance of the hallmark closed actin-binding cleft in these crystal structures is supported here by actin/S1-binding studies. These structures reveal how different duty ratios, and hence cellular functions, of the myosin isoforms may be accounted for, in part, on the basis of detailed differences in interdomain contacts. Moreover, the rigor-like position of switch II turns out to be unique for myosin V. The overall arrangements of subdomains in the motor are relatively conserved in each of the known contractile states, and we explore qualitatively the energetics of these states.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2OS8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Placopecten_magellanicus Placopecten magellanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OS8 OCA].
</div>
<div class="pdbe-citations 2os8" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor., Yang Y, Gourinath S, Kovacs M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Gyorgyi AG, Brown JH, Cohen C, Structure. 2007 May;15(5):553-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17502101 17502101]
*[[Myosin 3D Structures|Myosin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Placopecten magellanicus]]
[[Category: Placopecten magellanicus]]
[[Category: Protein complex]]
[[Category: Brown JH]]
[[Category: Brown, J H.]]
[[Category: Cohen C]]
[[Category: Cohen, C.]]
[[Category: Gourinath S]]
[[Category: Gourinath, S.]]
[[Category: Yang Y]]
[[Category: Yang, Y.]]
[[Category: Mechanical element]]
[[Category: Motor]]
[[Category: Myosin s1]]
[[Category: Rigor-like]]
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