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[[Image:2oo7.jpg|left|200px]]


{{Structure
==Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (T179I/Q199R)==
|PDB= 2oo7 |SIZE=350|CAPTION= <scene name='initialview01'>2oo7</scene>, resolution 1.800&Aring;
<StructureSection load='2oo7' size='340' side='right'caption='[[2oo7]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+920'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+B+921'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Residue+B+922'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+A+923'>AC4</scene>, <scene name='pdbsite=AC5:Ap5+Binding+Site+For+Residue+A+1218'>AC5</scene> and <scene name='pdbsite=AC6:Ap5+Binding+Site+For+Residue+B+1219'>AC6</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=AP5:BIS(ADENOSINE)-5&#39;-PENTAPHOSPHATE'>AP5</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2oo7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OO7 FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE= adk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AP5:BIS(ADENOSINE)-5-PENTAPHOSPHATE'>AP5</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oo7 OCA], [https://pdbe.org/2oo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oo7 RCSB], [https://www.ebi.ac.uk/pdbsum/2oo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oo7 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1p3j|1P3J]], [[2eu8|2EU8]], [[1zin|1ZIN]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oo7 OCA], [http://www.ebi.ac.uk/pdbsum/2oo7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oo7 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/KAD_BACSU KAD_BACSU] Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/2oo7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oo7 ConSurf].
<div style="clear:both"></div>


'''Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (T179I/Q199R)'''
==See Also==
 
*[[Adenylate kinase 3D structures|Adenylate kinase 3D structures]]
 
__TOC__
==About this Structure==
</StructureSection>
2OO7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OO7 OCA].
[[Category: Adenylate kinase]]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Counago, R.]]
[[Category: Counago R]]
[[Category: Myers, J C.]]
[[Category: Myers JC]]
[[Category: Shamoo, Y.]]
[[Category: Shamoo Y]]
[[Category: Wilson, C J.]]
[[Category: Wilson CJ]]
[[Category: Wittung-Stafshede, P.]]
[[Category: Wittung-Stafshede P]]
[[Category: Wu, G.]]
[[Category: Wu G]]
[[Category: adenylate kinase]]
[[Category: directed evolution]]
[[Category: experimental adaptive evolution]]
[[Category: thermostability]]
[[Category: transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:20:39 2008''

Latest revision as of 13:45, 30 August 2023

Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (T179I/Q199R)Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (T179I/Q199R)

Structural highlights

2oo7 is a 2 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KAD_BACSU Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2oo7, resolution 1.80Å

Drag the structure with the mouse to rotate

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OCA
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