2nxc: Difference between revisions

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-[[Image:2nxc.png|left|200px]]
-<!--
+==Apo-form of T. thermophilus ribosomal protein L11 methyltransferase (PrmA)==
-The line below this paragraph, containing "STRUCTURE_2nxc", creates the "Structure Box" on the page.
+<StructureSection load='2nxc' size='340' side='right'caption='[[2nxc]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2nxc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NXC FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nxc OCA], [https://pdbe.org/2nxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nxc RCSB], [https://www.ebi.ac.uk/pdbsum/2nxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nxc ProSAT]</span></td></tr>
-{{STRUCTURE_2nxc|  PDB=2nxc  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRMA_THET8 PRMA_THET8] Methylates ribosomal protein L11; this reaction probably occurs before the protein is assembled into the ribosome. This function is dispensable for growth and thermostability.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/2nxc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nxc ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacterial ribosomal protein L11 is post-translationally trimethylated at multiple residues by a single methyltransferase, PrmA. Here, we describe four structures of PrmA from the extreme thermophile Thermus thermophilus. Two apo-PrmA structures at 1.59 and 2.3 A resolution and a third with bound cofactor S-adenosyl-L-methionine at 1.75 A each exhibit distinct relative positions of the substrate recognition and catalytic domains, revealing how PrmA can position the L11 substrate for multiple, consecutive side-chain methylation reactions. The fourth structure, the PrmA-L11 enzyme-substrate complex at 2.4 A resolution, illustrates the highly specific interaction of the N-terminal domain with its substrate and places Lys39 in the PrmA active site. The presence of a unique flexible loop in the cofactor-binding site suggests how exchange of AdoMet with the reaction product S-adenosyl-L-homocysteine can occur without necessitating the dissociation of PrmA from L11. Finally, the mode of interaction of PrmA with L11 explains its observed preference for L11 as substrate before its assembly into the 50S ribosomal subunit.
-===Apo-form of T. thermophilus ribosomal protein L11 methyltransferase (PrmA)===
+Recognition of ribosomal protein L11 by the protein trimethyltransferase PrmA.,Demirci H, Gregory ST, Dahlberg AE, Jogl G EMBO J. 2007 Jan 24;26(2):567-77. Epub 2007 Jan 11. PMID:17215866<ref>PMID:17215866</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17215866}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2nxc" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17215866 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_17215866}}
-==About this Structure==
-[[2nxc]] is a 1 chain structure of [[Ribosomal protein L11 methyltransferase]] with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NXC OCA].
 ==See Also==
-*[[Ribosomal protein L11 methyltransferase]]
+*[[Ribosomal protein L11 methyltransferase|Ribosomal protein L11 methyltransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:17215866</ref><references group="xtra"/>
+__TOC__
-[[Category: Thermus thermophilus hb8]]
+</StructureSection>
-[[Category: Dahlberg, A E.]]
+[[Category: Large Structures]]
-[[Category: Demirci, H.]]
+[[Category: Thermus thermophilus HB8]]
-[[Category: Gregory, S T.]]
+[[Category: Dahlberg AE]]
-[[Category: Jogl, G.]]
+[[Category: Demirci H]]
-[[Category: Transferase s-adenosly-l-methionine dependent methyltransferase posttranslational modification]]
+[[Category: Gregory ST]]
+[[Category: Jogl G]]