2np7: Difference between revisions

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-[[Image:2np7.gif|left|200px]]
- {{Structure
+==Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position and pyrrolo-dC at the target base partner position==
-|PDB= 2np7 |SIZE=350|CAPTION= <scene name='initialview01'>2np7</scene>, resolution 1.90&Aring;
+<StructureSection load='2np7' size='340' side='right'caption='[[2np7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=NEA:5&#39;-DEOXY-5&#39;-[2-(AMINO)ETHYLTHIO]ADENOSINE'>NEA</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+<table><tr><td colspan='2'>[[2np7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NP7 FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-|GENE= taqIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=4PC:3-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-6-METHYL-3,7-DIHYDRO-2H-PYRROLO[2,3-D]PYRIMIDIN-2-ONE'>4PC</scene>, <scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NEA:5-DEOXY-5-[2-(AMINO)ETHYLTHIO]ADENOSINE'>NEA</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2np7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np7 OCA], [https://pdbe.org/2np7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2np7 RCSB], [https://www.ebi.ac.uk/pdbsum/2np7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2np7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTTA_THEAQ MTTA_THEAQ] This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TaqI endonuclease.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2np7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2np7 ConSurf].
+<div style="clear:both"></div>
-'''Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position and pyrrolo-dC at the target base partner position'''
+==See Also==
+*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
+__TOC__
-==About this Structure==
+</StructureSection>
-NP7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP7 OCA].
+[[Category: Large Structures]]
-[[Category: Single protein]]
-[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
 [[Category: Thermus aquaticus]]
-[[Category: Lenz, T.]]
+[[Category: Lenz T]]
-[[Category: Scheidig, A J.]]
+[[Category: Scheidig AJ]]
-[[Category: Weinhold, E.]]
+[[Category: Weinhold E]]
-[[Category: GOL]]
-[[Category: NEA]]
-[[Category: abasic site analog]]
-[[Category: base flipping]]
-[[Category: dna]]
-[[Category: dna methyltransferase]]
-[[Category: nucleotide flipping]]
-[[Category: pyrrolo-dc]]
-[[Category: target base partner]]
-[[Category: transferase/dna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:28:23 2008''