2ihe: Difference between revisions

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-[[Image:2ihe.png|left|200px]]
-{{STRUCTURE_2ihe|  PDB=2ihe  |  SCENE=  }}
+==Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus==
+<StructureSection load='2ihe' size='340' side='right'caption='[[2ihe]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ihe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IHE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ihe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ihe OCA], [https://pdbe.org/2ihe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ihe RCSB], [https://www.ebi.ac.uk/pdbsum/2ihe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ihe ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SSB_THEAQ SSB_THEAQ]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/2ihe_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ihe ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In contrast to the majority of tetrameric SSB proteins, the recently discovered SSB proteins from the Thermus/Deinoccus group form dimers. We solved the crystal structures of the SSB protein from Thermus aquaticus (TaqSSB) and a deletion mutant of the protein and show the structure of their ssDNA binding domains to be similar to the structure of tetrameric SSBs. Two conformations accompanied by proline cis-trans isomerization are observed in the flexible C-terminal region. For the first time, we were able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein. This highly conserved region is essential for interaction with other proteins and we show it to adopt an extended conformation devoid of secondary structure. A model for binding this region to the chi subunit of DNA polymerase III is proposed. It explains at a molecular level the reason for the ssb113 phenotype observed in Escherichia coli.
-===Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus===
+D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.,Fedorov R, Witte G, Urbanke C, Manstein DJ, Curth U Nucleic Acids Res. 2006;34(22):6708-17. Epub 2006 Dec 5. PMID:17148487<ref>PMID:17148487</ref>
-{{ABSTRACT_PUBMED_17148487}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ihe" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[2ihe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHE OCA].
+*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:017148487</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Thermus aquaticus]]
-[[Category: Curth, U.]]
+[[Category: Curth U]]
-[[Category: Fedorov, R.]]
+[[Category: Fedorov R]]
-[[Category: Manstein, D J.]]
+[[Category: Manstein DJ]]
-[[Category: Urbanke, C.]]
+[[Category: Urbanke C]]
-[[Category: Witte, G.]]
+[[Category: Witte G]]
-[[Category: Dna binding protein]]
-[[Category: Protein-dna interaction]]
-[[Category: Protein-protein interaction]]
-[[Category: Thermophile organism]]