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[[Image:2han.gif|left|200px]]


{{Structure
==Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter==
|PDB= 2han |SIZE=350|CAPTION= <scene name='initialview01'>2han</scene>, resolution 1.95&Aring;
<StructureSection load='2han' size='340' side='right'caption='[[2han]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2han]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HAN FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
|GENE= usp, Cf1, NR2B4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]), EcR, NR1H1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2han FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2han OCA], [https://pdbe.org/2han PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2han RCSB], [https://www.ebi.ac.uk/pdbsum/2han PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2han ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2han FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2han OCA], [http://www.ebi.ac.uk/pdbsum/2han PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2han RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/USP_DROME USP_DROME] Receptor for ecdysone. May be an important modulator of insect metamorphosis. Plays an important part in embryonic and post-embryonic development. Binds to ecdysone response elements (ECRES) such as in the promoter region of s15 chorion gene.
 
== Evolutionary Conservation ==
'''Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2han_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2han ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the functional receptor for ecdysteroids initiating molting and metamorphosis in insects. Here we report the 1.95 A structure of the complex formed by the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural pseudopalindromic response element. Comparison of the structure with that obtained previously, using an idealized response element, shows how the EcRDBD, which has been previously reported to possess extraordinary flexibility, accommodates DNA-induced structural changes. Part of the C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix whose location in the minor groove does not match any of the locations previously observed for nuclear receptors. Mutational analyses suggest that the alpha-helix is a component of EcR-box, a novel element indispensable for DNA-binding and located within the nuclear receptor CTE. This element seems to be a general feature of all known EcRs.
The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the functional receptor for ecdysteroids initiating molting and metamorphosis in insects. Here we report the 1.95 A structure of the complex formed by the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural pseudopalindromic response element. Comparison of the structure with that obtained previously, using an idealized response element, shows how the EcRDBD, which has been previously reported to possess extraordinary flexibility, accommodates DNA-induced structural changes. Part of the C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix whose location in the minor groove does not match any of the locations previously observed for nuclear receptors. Mutational analyses suggest that the alpha-helix is a component of EcR-box, a novel element indispensable for DNA-binding and located within the nuclear receptor CTE. This element seems to be a general feature of all known EcRs.


==About this Structure==
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.,Jakob M, Kolodziejczyk R, Orlowski M, Krzywda S, Kowalska A, Dutko-Gwozdz J, Gwozdz T, Kochman M, Jaskolski M, Ozyhar A Nucleic Acids Res. 2007;35(8):2705-18. Epub 2007 Apr 10. PMID:17426125<ref>PMID:17426125</ref>
2HAN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HAN OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain., Jakob M, Kolodziejczyk R, Orlowski M, Krzywda S, Kowalska A, Dutko-Gwozdz J, Gwozdz T, Kochman M, Jaskolski M, Ozyhar A, Nucleic Acids Res. 2007;35(8):2705-18. Epub 2007 Apr 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17426125 17426125]
</div>
<div class="pdbe-citations 2han" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Dutko-Gwozdz, J.]]
[[Category: Dutko-Gwozdz J]]
[[Category: Gwozdz, T.]]
[[Category: Gwozdz T]]
[[Category: Jakob, M.]]
[[Category: Jakob M]]
[[Category: Jaskolski, M.]]
[[Category: Jaskolski M]]
[[Category: Kochman, M.]]
[[Category: Kochman M]]
[[Category: Kolodziejczyk, R.]]
[[Category: Kolodziejczyk R]]
[[Category: Kowalska, A.]]
[[Category: Kowalska A]]
[[Category: Krzywda, S.]]
[[Category: Krzywda S]]
[[Category: Orlowski, M.]]
[[Category: Orlowski M]]
[[Category: Ozyhar, A.]]
[[Category: Ozyhar A]]
[[Category: dna-binding]]
[[Category: nuclear protein]]
[[Category: nuclear receptor]]
[[Category: transcription factor]]
[[Category: transcription regulation]]
[[Category: zinc finger]]
 
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