2gsq: Difference between revisions

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-[[Image:2gsq.png|left|200px]]
-{{STRUCTURE_2gsq|  PDB=2gsq  |  SCENE=  }}
+==GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE==
+<StructureSection load='2gsq' size='340' side='right'caption='[[2gsq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2gsq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nototodarus_sloanii Nototodarus sloanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GSQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GBI:S-(3-IODOBENZYL)GLUTATHIONE'>GBI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsq OCA], [https://pdbe.org/2gsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gsq RCSB], [https://www.ebi.ac.uk/pdbsum/2gsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gsq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GST_NOTSL GST_NOTSL] High activity toward 1-chloro-2,4-dinitrobenzene. Not very efficient at catalyzing the addition of GSH to enones and epoxides.<ref>PMID:7727393</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/2gsq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gsq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
-===GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE===
+Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.,Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848<ref>PMID:8710848</ref>
-{{ABSTRACT_PUBMED_8710848}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2gsq" style="background-color:#fffaf0;"></div>
-[[2gsq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nototodarus_sloanii Nototodarus sloanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA].
 ==See Also==
-*[[Glutathione S-transferase|Glutathione S-transferase]]
+*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:008710848</ref><references group="xtra"/>
+__TOC__
-[[Category: Glutathione transferase]]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Nototodarus sloanii]]
-[[Category: Armstrong, R N.]]
+[[Category: Armstrong RN]]
-[[Category: Gilliland, G L.]]
+[[Category: Gilliland GL]]
-[[Category: Ji, X.]]
+[[Category: Ji X]]
-[[Category: Sigma class]]
-[[Category: Squid digestive gland]]
-[[Category: Transferase]]