2gg4: Difference between revisions

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{{Seed}}
[[Image:2gg4.png|left|200px]]


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==CP4 EPSP synthase (unliganded)==
The line below this paragraph, containing "STRUCTURE_2gg4", creates the "Structure Box" on the page.
<StructureSection load='2gg4' size='340' side='right'caption='[[2gg4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gg4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_sp._CP4 Agrobacterium sp. CP4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GG4 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gg4 OCA], [https://pdbe.org/2gg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gg4 RCSB], [https://www.ebi.ac.uk/pdbsum/2gg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gg4 ProSAT]</span></td></tr>
{{STRUCTURE_2gg4|  PDB=2gg4  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROA_AGRSC AROA_AGRSC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gg/2gg4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gg4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation.


===CP4 EPSP synthase (unliganded)===
Molecular basis for the herbicide resistance of Roundup Ready crops.,Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934<ref>PMID:16916934</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2gg4" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_16916934}}, adds the Publication Abstract to the page
*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 16916934 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_16916934}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Agrobacterium sp. CP4]]
2GG4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_sp. Agrobacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GG4 OCA].
[[Category: Large Structures]]
 
[[Category: Funke T]]
==Reference==
[[Category: Schonbrunn E]]
<ref group="xtra">PMID:16916934</ref><references group="xtra"/>
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
[[Category: Agrobacterium sp.]]
[[Category: Funke, T.]]
[[Category: Schonbrunn, E.]]
[[Category: Inside-out alpha/beta barrel]]
[[Category: Two domain structure]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:04:08 2009''

Latest revision as of 12:40, 30 August 2023

CP4 EPSP synthase (unliganded)CP4 EPSP synthase (unliganded)

Structural highlights

2gg4 is a 1 chain structure with sequence from Agrobacterium sp. CP4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AROA_AGRSC

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation.

Molecular basis for the herbicide resistance of Roundup Ready crops.,Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E. Molecular basis for the herbicide resistance of Roundup Ready crops. Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934

2gg4, resolution 2.10Å

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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