2fy8: Difference between revisions

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-[[Image:2fy8.gif|left|200px]]
- {{Structure
+==Crystal structure of MthK rck domain in its ligand-free gating-ring form==
-|PDB= 2fy8 |SIZE=350|CAPTION= <scene name='initialview01'>2fy8</scene>, resolution 2.79&Aring;
+<StructureSection load='2fy8' size='340' side='right'caption='[[2fy8]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[2fy8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FY8 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.79&#8491;</td></tr>
-|GENE= mthK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fy8 OCA], [https://pdbe.org/2fy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fy8 RCSB], [https://www.ebi.ac.uk/pdbsum/2fy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fy8 ProSAT]</span></td></tr>
-}}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/2fy8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fy8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+MthK is a prokaryotic Ca(2+)-gated K(+) channel that, like other ligand-gated channels, converts the chemical energy of ligand binding to the mechanical force of channel opening. The channel's eight ligand-binding domains, the RCK domains, form an octameric gating ring in which Ca(2+) binding induces conformational changes that open the channel. Here we present the crystal structures of the MthK gating ring in closed and partially open states at 2.8 A, both obtained from the same crystal grown in the absence of Ca(2+). Furthermore, our biochemical and electrophysiological analyses demonstrate that MthK is regulated by both Ca(2+) and pH. Ca(2+) regulates the channel by changing the equilibrium of the gating ring between closed and open states, while pH regulates channel gating by affecting gating-ring stability. Our findings, along with the previously determined open MthK structure, allow us to elucidate the ligand gating mechanism of RCK-regulated K(+) channels.
-'''Crystal structure of MthK rck domain in its ligand-free gating-ring form'''
+Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels.,Ye S, Li Y, Chen L, Jiang Y Cell. 2006 Sep 22;126(6):1161-73. PMID:16990139<ref>PMID:16990139</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2fy8" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-MthK is a prokaryotic Ca(2+)-gated K(+) channel that, like other ligand-gated channels, converts the chemical energy of ligand binding to the mechanical force of channel opening. The channel's eight ligand-binding domains, the RCK domains, form an octameric gating ring in which Ca(2+) binding induces conformational changes that open the channel. Here we present the crystal structures of the MthK gating ring in closed and partially open states at 2.8 A, both obtained from the same crystal grown in the absence of Ca(2+). Furthermore, our biochemical and electrophysiological analyses demonstrate that MthK is regulated by both Ca(2+) and pH. Ca(2+) regulates the channel by changing the equilibrium of the gating ring between closed and open states, while pH regulates channel gating by affecting gating-ring stability. Our findings, along with the previously determined open MthK structure, allow us to elucidate the ligand gating mechanism of RCK-regulated K(+) channels.
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-FY8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FY8 OCA].
+__TOC__
+</StructureSection>
-==Reference==
+[[Category: Large Structures]]
-Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels., Ye S, Li Y, Chen L, Jiang Y, Cell. 2006 Sep 22;126(6):1161-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16990139 16990139]
 [[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Single protein]]
+[[Category: Jiang Y]]
-[[Category: Jiang, Y.]]
+[[Category: Ye S]]
-[[Category: Ye, S.]]
-[[Category: 3d-structure]]
-[[Category: alternative initiation]]
-[[Category: calcium]]
-[[Category: closed conformation]]
-[[Category: gating ring]]
-[[Category: ion transport]]
-[[Category: ionic channel]]
-[[Category: membrane]]
-[[Category: partially open conformation]]
-[[Category: potassium]]
-[[Category: potassium transport]]
-[[Category: transition state]]
-[[Category: transmembrane]]
-[[Category: transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:58:15 2008''