2fdh: Difference between revisions
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<StructureSection load='2fdh' size='340' side='right'caption='[[2fdh]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2fdh' size='340' side='right'caption='[[2fdh]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2fdh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2fdh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FDH FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=MA7:1N-METHYLADENOSINE-5-MONOPHOSPHATE'>MA7</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fdh OCA], [https://pdbe.org/2fdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fdh RCSB], [https://www.ebi.ac.uk/pdbsum/2fdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fdh ProSAT], [https://www.topsan.org/Proteins/NESGC/2fdh TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fdh OCA], [https://pdbe.org/2fdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fdh RCSB], [https://www.ebi.ac.uk/pdbsum/2fdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fdh ProSAT], [https://www.topsan.org/Proteins/NESGC/2fdh TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/ALKB_ECOLI ALKB_ECOLI] Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).<ref>PMID:12226668</ref> <ref>PMID:12594517</ref> <ref>PMID:16482161</ref> <ref>PMID:19706517</ref> <ref>PMID:21068844</ref> <ref>PMID:20084272</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli K-12]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Benach | [[Category: Benach J]] | ||
[[Category: Edstrom | [[Category: Edstrom WC]] | ||
[[Category: Gibney | [[Category: Gibney BR]] | ||
[[Category: Hunt | [[Category: Hunt JF]] | ||
[[Category: Yu B]] | |||
[[Category: Yu | |||