Pheromone binding protein: Difference between revisions

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Nurit Eliash, Michal Harel

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+<StructureSection load='3bfa' size='340' side='right' caption='Pheromone binding protein of honey bee complex with pheromone (PDB code [[3bfa]]).' scene=''>
+__TOC__
 ==Introduction==
-<StructureSection load='3bfa' size='340' side='right' caption='Pheromone binding protein of ''Apis mellifera'' scene=''>
+'''Pheromone binding proteins''' [http://en.wikipedia.org/wiki/Pheromone_binding_protein (PBP)] are type of Odorant binding proteins [http://en.wikipedia.org/wiki/Odorant-binding_protein (OBP)] - soluble proteins mediating the early stages of volatiles detection in both insects and vertebrates<ref>DOI:10.3389/fphys.2014.00320</ref>. The volatiles (pheromones and other small hydrophobic molecules) are solubilized by the OBPs and activate the chemoreceptors.
-Pheromone binding proteins are soluble proteins invovled in the early stages of pheromone detection.
-As a model protein of this family we will further describe the well investigated Pheromone binding protein of the honey bee.
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+As a model protein of this family we will further describe the well investigated Pheromone binding protein of the honey bee, ASP1.
-Pheromone-binding protein [http://www.uniprot.org/uniprot/Q9U9J6/ ASP] of the honeybee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] L. (Hymenoptera: Apidea) was first isolated and charecterised by Danty et al. (1998)<ref>DOI:10.1016/j.jmb.2008.04.048</ref> from the bee antennae.
+== Pheromone-binding protein ASP1 ==
-or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
+Chemical communication is crucial in social insects, where a complicated and delicate system of signals must be maintained in order to preserve the fragile equilibrium between the queen and the workers. In the hive of the honey bee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] this equilibrium exists partially due to the extraction of blend of substances called queen mandibular pheromone [http://en.wikipedia.org/wiki/Honey_bee_pheromones#Queen_mandibular_pheromone (QMP)], by the queen <ref>Winston, M.L., 1987. The Biology of the Honey Bee. Harvard University Press, Cambridge, MA.</ref>. The three major component of the QMP blend are: 9-keto-2(E)-decenoic acid (9-ODA) and 9-hydroxy-2(E)-decenoic acid (9-HDA R-(−) or S-(+)).
+Pheromone-binding protein [http://www.uniprot.org/uniprot/Q9U9J6/ ASP1] of the honeybee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] L. (Hymenoptera: Apidea) was first isolated and characterized by Danty ''et al''. (1998)<ref>DOI:10.1016/j.jmb.2008.04.048</ref> from the bee antennae.
 == Structure ==
-[[3bfa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFA OCA].
+The protein is composed of 144 amino acids, which forms 6 alpha helices. Three <scene name='60/609542/Disulfide_bonds/1'>3 disulfide bonds</scene> formed by 6 Cystein residues tied four helices: disulfide 20–51 between H1 and H3, 47– 98 between H3 and H6, and 107–89 between H6 and H5.
-== Function ==
+== Interaction with the ligand 9-ODA==
+One of the main components of the QMP <scene name='60/609542/9-oda/3'>9-ODA</scene>, is binding to the protein binding site along with a <scene name='60/609542/Glycerol/2'>glycerol molecule</scene>.The carboxyl end of 9-ODA points towards the solvent, and has no bonds with residues of the protein. The residues in the binding site are <scene name='60/609542/Binding_site/3'>hydrophobic</scene>, and the connection between 9-ODA and ASP1 involve hydrogen bonds.
-== Disease ==
+</StructureSection>
+==3D structures of pheromone-binding protein==
+Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+* Pheromone binding protein
-== Relevance ==
+**[[2h8v]], [[3bjh]], [[3cab]], [[3cdn]], [[3cz2]] – bPBP residues 26-144 – honey bee<br />
+**[[3d73]], [[3d74]], [[3d75]], [[3d76]], [[3d77]], [[3d78]] - bPBP residues 26-144 (mutant)<br />
+**[[1dqe]], [[2fjy]] – sPBP – silkworm<br />
+**[[1xfr]] – sPBP – NMR<br />
+**[[1gm0]] – sPBP (mutant) – NMR<br />
+**[[1qwv]], [[1two]], [[2jpo]], [[6um9]] – mPBP – moth - NMR<br />
+**[[6vq5]] – mPBP <br />
+**[[7uo6]] – PBP2 – corn borer - NMR<br />
+**[[7vw8]], [[7vw9]] – bwPBP1 - bollworm<br />
-== Structural highlights ==
+* Pheromone binding protein complex
-This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
+**[[3bfa]], [[3bfb]], [[3bfh]], [[3cyz]] – bPBP residues 26-144 + pheromone<br />
+**[[3cz0]], [[3cz1]] - bPBP residues 26-144 + N-butyl benzene sulfonamide<br />
+**[[3fe6]], [[3fe8]], [[3fe9]] - bPBP residues 26-144 + methyldotetracontane<br />
+**[[2p70]] – sPBP + odorant<br />
+**[[2p71]] – sPBP + iodohexadecane<br />
+**[[4inw]], [[4inx]] – PBP + hexadecadienal – ''Amyelois transitella''<br />
+**[[7vwa]] – bwPBP1 + odorant <br />
+}}
-</StructureSection>
 == References ==
 <references/>
+[[Category: Topic Page]]