5i6c: Difference between revisions

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-'''Unreleased structure'''
-The entry 5i6c is ON HOLD  until Paper Publication
+==The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine==
+<StructureSection load='5i6c' size='340' side='right'caption='[[5i6c]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5i6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I6C FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=XAN:XANTHINE'>XAN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i6c OCA], [https://pdbe.org/5i6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i6c RCSB], [https://www.ebi.ac.uk/pdbsum/5i6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i6c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/UAPA_EMENI UAPA_EMENI] Uric acid-xanthine transporter.<ref>PMID:15953615</ref> <ref>PMID:16096268</ref> <ref>PMID:20002879</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The uric acid/xanthine H(+) symporter, UapA, is a high-affinity purine transporter from the filamentous fungus Aspergillus nidulans. Here we present the crystal structure of a genetically stabilized version of UapA (UapA-G411VDelta1-11) in complex with xanthine. UapA is formed from two domains, a core domain and a gate domain, similar to the previously solved uracil transporter UraA, which belongs to the same family. The structure shows UapA in an inward-facing conformation with xanthine bound to residues in the core domain. Unlike UraA, which was observed to be a monomer, UapA forms a dimer in the crystals with dimer interactions formed exclusively through the gate domain. Analysis of dominant negative mutants is consistent with dimerization playing a key role in transport. We postulate that UapA uses an elevator transport mechanism likely to be shared with other structurally homologous transporters including anion exchangers and prestin.
-Authors: Alguel, Y., Amillis, S., Leung, J., Lambrinidis, G., Capaldi, S., Scull, N.J., Craven, G., Iwata, S., Armstrong, A., Mikros, E., Diallinas, G., Cameron, A.D., Byrne, B.
+Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.,Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B Nat Commun. 2016 Apr 18;7:11336. doi: 10.1038/ncomms11336. PMID:27088252<ref>PMID:27088252</ref>
-Description: The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Alguel, Y]]
+<div class="pdbe-citations 5i6c" style="background-color:#fffaf0;"></div>
-[[Category: Iwata, S]]
-[[Category: Mikros, E]]
+==See Also==
-[[Category: Capaldi, S]]
+*[[Symporter 3D structures|Symporter 3D structures]]
-[[Category: Amillis, S]]
+== References ==
-[[Category: Scull, N.J]]
+<references/>
-[[Category: Leung, J]]
+__TOC__
-[[Category: Byrne, B]]
+</StructureSection>
-[[Category: Cameron, A.D]]
+[[Category: Aspergillus nidulans FGSC A4]]
-[[Category: Craven, G]]
+[[Category: Large Structures]]
-[[Category: Diallinas, G]]
+[[Category: Alguel Y]]
-[[Category: Armstrong, A]]
+[[Category: Amillis S]]
-[[Category: Lambrinidis, G]]
+[[Category: Armstrong A]]
+[[Category: Byrne B]]
+[[Category: Cameron AD]]
+[[Category: Capaldi S]]
+[[Category: Craven G]]
+[[Category: Diallinas G]]
+[[Category: Iwata S]]
+[[Category: Lambrinidis G]]
+[[Category: Leung J]]
+[[Category: Mikros E]]
+[[Category: Scull NJ]]