2y9e: Difference between revisions

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-[[Image:2y9e.png|left|200px]]
-{{STRUCTURE_2y9e|  PDB=2y9e  |  SCENE=  }}
+==Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2==
+<StructureSection load='2y9e' size='340' side='right'caption='[[2y9e]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2y9e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y9E FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.397&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9e OCA], [https://pdbe.org/2y9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y9e RCSB], [https://www.ebi.ac.uk/pdbsum/2y9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9e ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures.
-===STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2===
+Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref>
-{{ABSTRACT_PUBMED_21841195}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2y9e" style="background-color:#fffaf0;"></div>
-[[2y9e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9E OCA].
 ==See Also==
-*[[Myosin|Myosin]]
+*[[Myosin 3D Structures|Myosin 3D Structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021841195</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Dictyostelium discoideum]]
-[[Category: Myosin ATPase]]
+[[Category: Large Structures]]
-[[Category: Adamek, N.]]
+[[Category: Adamek N]]
-[[Category: Bauer, S.]]
+[[Category: Bauer S]]
-[[Category: Fedorov, R.]]
+[[Category: Fedorov R]]
-[[Category: Fujita-Becker, S.]]
+[[Category: Fujita-Becker S]]
-[[Category: Geeves, M A.]]
+[[Category: Geeves MA]]
-[[Category: Manstein, D J.]]
+[[Category: Manstein DJ]]
-[[Category: Preller, M.]]
+[[Category: Preller M]]
-[[Category: Motor protein]]