2alx: Difference between revisions

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OCA

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-[[Image:2alx.gif|left|200px]]<br /><applet load="2alx" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2alx, resolution 2.600&Aring;" />
-'''Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22'''<br />
-==Overview==
+==Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22==
-A new crystal form of wild-type ribonucleotide reductase R2 from, Escherichia coli was obtained. Crystals grow in space group P6(1)22 with, one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry, axis generates the physiological dimeric form of R2. Co-crystallization, with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal, site. The structure of the Mn(II)-loaded form was determined to 2.6, Angstroms resolution by molecular replacement. The crystallization, conditions, backbone conformation, crystal-packing interactions and metal, centers are compared with those of previously determined crystal forms.
+<StructureSection load='2alx' size='340' side='right'caption='[[2alx]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2alx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ALX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2alx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2alx OCA], [https://pdbe.org/2alx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2alx RCSB], [https://www.ebi.ac.uk/pdbsum/2alx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2alx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RIR2_ECOLI RIR2_ECOLI] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/al/2alx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2alx ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
-==About this Structure==
+Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122.,Sommerhalter M, Saleh L, Bollinger JM Jr, Rosenzweig AC Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1649-54. Epub 2005, Nov 19. PMID:16301799<ref>PMID:16301799</ref>
-ALX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and HG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ALX OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122., Sommerhalter M, Saleh L, Bollinger JM Jr, Rosenzweig AC, Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1649-54. Epub 2005, Nov 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16301799 16301799]
+</div>
+<div class="pdbe-citations 2alx" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribonucleotide reductase 3D structures|Ribonucleotide reductase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Ribonucleoside-diphosphate reductase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Bollinger Jr JM]]
-[[Category: Jr., J.M.Bollinger.]]
+[[Category: Rosenzweig AC]]
-[[Category: Rosenzweig, A.C.]]
+[[Category: Saleh L]]
-[[Category: Saleh, L.]]
+[[Category: Sommerhalter M]]
-[[Category: Sommerhalter, M.]]
-[[Category: HG]]
-[[Category: MN]]
-[[Category: diiron center]]
-[[Category: dimanganese center]]
-[[Category: new crystal form]]
-[[Category: ribonucleotide reductase r2]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:12:48 2007''