2a4n: Difference between revisions

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-[[Image:2a4n.png|left|200px]]
-<!--
+==Crystal structure of aminoglycoside 6'-N-acetyltransferase complexed with coenzyme A==
-The line below this paragraph, containing "STRUCTURE_2a4n", creates the "Structure Box" on the page.
+<StructureSection load='2a4n' size='340' side='right'caption='[[2a4n]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2a4n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4N FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2a4n|  PDB=2a4n  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4n OCA], [https://pdbe.org/2a4n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4n RCSB], [https://www.ebi.ac.uk/pdbsum/2a4n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4n ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q47764_ENTFC Q47764_ENTFC]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a4n ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The aminoglycoside-modifying enzyme aminoglycoside 6'-N-acetyltransferase type Ii [AAC(6')-Ii] has been crystallized with its cofactor coenzyme A in space group C222(1), with unit-cell parameters a = 71.5, b = 127.4, c = 76.9 A and one physiologically relevant dimer species per asymmetric unit. The space group previously observed for this complex was P2(1)2(1)2(1), with two dimers per asymmetric unit. By comparing the six available protomer structures of the AAC(6')-Ii-CoA complex, it has been possible to identify regions of plasticity within the protein. Normal-mode analysis of this complex suggests that this plasticity is not an artefact of crystal-packing forces, but that the region of the protomer that displays multiple conformations is intrinsically flexible. It is conjectured that the flexibility is relevant for the cooperative activity observed for the enzyme.
-===Crystal structure of aminoglycoside 6'-N-acetyltransferase complexed with coenzyme A===
+Structures of aminoglycoside acetyltransferase AAC(6')-Ii in a novel crystal form: structural and normal-mode analyses.,Burk DL, Xiong B, Breitbach C, Berghuis AM Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1273-9. Epub 2005, Aug 16. PMID:16131761<ref>PMID:16131761</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_16131761}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2a4n" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 16131761 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16131761}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[2a4n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4N OCA].
-==Reference==
-<ref group="xtra">PMID:016131761</ref><references group="xtra"/>
 [[Category: Enterococcus faecium]]
-[[Category: Berghuis, A M.]]
+[[Category: Large Structures]]
-[[Category: Breitbach, C.]]
+[[Category: Berghuis AM]]
-[[Category: Burk, D L.]]
+[[Category: Breitbach C]]
-[[Category: Xiong, B.]]
+[[Category: Burk DL]]
-[[Category: Alpha beta protein]]
+[[Category: Xiong B]]
-[[Category: N-acetyl transferase]]
-[[Category: Transferase]]