1zs2: Difference between revisions

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[[Image:1zs2.gif|left|200px]]


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==Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose==
The line below this paragraph, containing "STRUCTURE_1zs2", creates the "Structure Box" on the page.
<StructureSection load='1zs2' size='340' side='right'caption='[[1zs2]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1zs2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZS2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
{{STRUCTURE_1zs2| PDB=1zs2 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zs2 OCA], [https://pdbe.org/1zs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zs2 RCSB], [https://www.ebi.ac.uk/pdbsum/1zs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zs2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/1zs2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zs2 ConSurf].
<div style="clear:both"></div>


'''Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose'''
==See Also==
 
*[[Amylase 3D structures|Amylase 3D structures]]
 
== References ==
==About this Structure==
<references/>
1ZS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS2 OCA].
__TOC__
[[Category: Amylosucrase]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria polysaccharea]]
[[Category: Neisseria polysaccharea]]
[[Category: Single protein]]
[[Category: Albenne C]]
[[Category: Albenne, C.]]
[[Category: Gajhede M]]
[[Category: Gajhede, M.]]
[[Category: Mirza O]]
[[Category: Mirza, O.]]
[[Category: Monsan P]]
[[Category: Monsan, P.]]
[[Category: Remaud-Simeon M]]
[[Category: Remaud-Simeon, M.]]
[[Category: Skov LK]]
[[Category: Skov, L K.]]
[[Category: Sprogoe D]]
[[Category: Sprogoe, D.]]
[[Category: Van der Veen BA]]
[[Category: Veen, B A.van der.]]
[[Category: Ternary complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:00:01 2008''

Latest revision as of 10:13, 23 August 2023

Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaoseAmylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose

Structural highlights

1zs2 is a 1 chain structure with sequence from Neisseria polysaccharea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.16Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AMYS_NEIPO Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ De Montalk GP, Remaud-Simeon M, Willemot RM, Planchot V, Monsan P. Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme. J Bacteriol. 1999 Jan;181(2):375-81. PMID:9882648

1zs2, resolution 2.16Å

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