1xn4: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1xn4.png|left|200px]]
-<!--
+==PUTATIVE MAR1 RIBONUCLEASE FROM LEISHMANIA MAJOR==
-The line below this paragraph, containing "STRUCTURE_1xn4", creates the "Structure Box" on the page.
+<StructureSection load='1xn4' size='340' side='right'caption='[[1xn4]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1xn4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XN4 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xn4 OCA], [https://pdbe.org/1xn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xn4 RCSB], [https://www.ebi.ac.uk/pdbsum/1xn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xn4 ProSAT]</span></td></tr>
-{{STRUCTURE_1xn4|  PDB=1xn4  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q4QGT7_LEIMA Q4QGT7_LEIMA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xn4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xn4 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have determined the crystal structures of three homologous proteins from the pathogenic protozoans Leishmania donovani, Leishmania major, and Trypanosoma cruzi. We propose that these proteins represent a new subfamily within the isochorismatase superfamily (CDD classification cd004310). Their overall fold and key active site residues are structurally homologous both to the biochemically well-characterized N-carbamoylsarcosine-amidohydrolase, a cysteine hydrolase, and to the phenazine biosynthesis protein PHZD (isochorismase), an aspartyl hydrolase. All three proteins are annotated as mitochondrial-associated ribonuclease Mar1, based on a previous characterization of the homologous protein from L. tarentolae. This would constitute a new enzymatic activity for this structural superfamily, but this is not strongly supported by the observed structures. In these protozoan proteins, the extended active site is formed by inter-subunit association within a tetramer, which implies a distinct evolutionary history and substrate specificity from the previously characterized members of the isochorismatase superfamily. The characterization of the active site is supported crystallographically by the presence of an unidentified ligand bound at the active site cysteine of the T. cruzi structure.
-===PUTATIVE MAR1 RIBONUCLEASE FROM LEISHMANIA MAJOR===
+Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily.,Caruthers J, Zucker F, Worthey E, Myler PJ, Buckner F, Van Voorhuis W, Mehlin C, Boni E, Feist T, Luft J, Gulde S, Lauricella A, Kaluzhniy O, Anderson L, Le Trong I, Holmes MA, Earnest T, Soltis M, Hodgson KO, Hol WG, Merritt EA Protein Sci. 2005 Nov;14(11):2887-94. Epub 2005 Sep 30. PMID:16199669<ref>PMID:16199669</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_016199669}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1xn4" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 016199669 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_016199669}}
-==About this Structure==
-[[1xn4]] is a 1 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XN4 OCA].
 ==See Also==
-*[[Ribonuclease|Ribonuclease]]
+*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:016199669</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Leishmania major]]
-[[Category: Caruthers, J.]]
+[[Category: Caruthers J]]
-[[Category: Merritt, E A.]]
+[[Category: Merritt EA]]
-[[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Sgpp]]
-[[Category: Structural genomic]]
-[[Category: Structural genomics of pathogenic protozoa consortium]]
-[[Category: Unknown function]]