1ssq: Difference between revisions

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-[[Image:1ssq.png|left|200px]]
-{{STRUCTURE_1ssq|  PDB=1ssq  |  SCENE=  }}
+==Serine Acetyltransferase- Complex with Cysteine==
+<StructureSection load='1ssq' size='340' side='right'caption='[[1ssq]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ssq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SSQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ssq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ssq OCA], [https://pdbe.org/1ssq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ssq RCSB], [https://www.ebi.ac.uk/pdbsum/1ssq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ssq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CYSE_HAEIN CYSE_HAEIN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ss/1ssq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ssq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Serine acetyltransferase (SAT, EC 2.3.1.30) catalyzes the CoA-dependent acetylation of the side chain hydroxyl group of l-serine to form O-acetylserine, as the first step of a two-step biosynthetic pathway in bacteria and plants leading to the formation of l-cysteine. This reaction represents a key metabolic point of regulation for the cysteine biosynthetic pathway due to its feedback inhibition by cysteine. We have determined the X-ray crystal structure of Haemophilus influenzae SAT in complexes with CoA and its cysteine feedback inhibitor. The enzyme is a 175 kDa hexamer displaying the characteristic left-handed parallel beta-helix (LbetaH) structural domain of the hexapeptide acyltransferase superfamily of enzymes. Cysteine is bound in a crevice between adjacent LbetaH domains and underneath a loop excluded from the coiled LbetaH. The proximity of its thiol group to the thiol group of CoA derived from superimposed models of the cysteine and CoA complexes confirms that cysteine is bound at the active site. Analysis of the contacts of SAT with cysteine and CoA and the conformational differences that distinguish these complexes provides a structural basis for cysteine feedback inhibition, which invokes competition between cysteine and serine binding and a cysteine-induced conformational change of the C-terminal segment of the enzyme that excludes binding of the cofactor.
-===Serine Acetyltransferase- Complex with Cysteine===
+Structure of serine acetyltransferase in complexes with CoA and its cysteine feedback inhibitor.,Olsen LR, Huang B, Vetting MW, Roderick SL Biochemistry. 2004 May 25;43(20):6013-9. PMID:15147185<ref>PMID:15147185</ref>
-{{ABSTRACT_PUBMED_15147185}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1ssq" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[1ssq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSQ OCA].
+*[[Serine acetyltransferase|Serine acetyltransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:015147185</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Haemophilus influenzae]]
-[[Category: Serine O-acetyltransferase]]
+[[Category: Large Structures]]
-[[Category: Huang, B.]]
+[[Category: Huang B]]
-[[Category: Olsen, L R.]]
+[[Category: Olsen LR]]
-[[Category: Roderick, S L.]]
+[[Category: Roderick SL]]
-[[Category: Vetting, M W.]]
+[[Category: Vetting MW]]
-[[Category: Left-handed parallel beta helix]]
-[[Category: Transferase]]