1sr6: Difference between revisions

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-[[Image:1sr6.png|left|200px]]
-<!--
+==Structure of nucleotide-free scallop myosin S1==
-The line below this paragraph, containing "STRUCTURE_1sr6", creates the "Structure Box" on the page.
+<StructureSection load='1sr6' size='340' side='right'caption='[[1sr6]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1sr6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SR6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_1sr6|  PDB=1sr6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr6 OCA], [https://pdbe.org/1sr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sr6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MYS_ARGIR MYS_ARGIR] Muscle contraction.  Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Structural studies of myosin have indicated some of the conformational changes that occur in this protein during the contractile cycle, and we have now observed a conformational change in a bound nucleotide as well. The 3.1-A x-ray structure of the scallop myosin head domain (subfragment 1) in the ADP-bound near-rigor state (lever arm =45 degrees to the helical actin axis) shows the diphosphate moiety positioned on the surface of the nucleotide-binding pocket, rather than deep within it as had been observed previously. This conformation strongly suggests a specific mode of entry and exit of the nucleotide from the nucleotide-binding pocket through the so-called "front door." In addition, using a variety of scallop structures, including a relatively high-resolution 2.75-A nucleotide-free near-rigor structure, we have identified a conserved complex salt bridge connecting the 50-kDa upper and N-terminal subdomains. This salt bridge is present only in crystal structures of muscle myosin isoforms that exhibit a strong reciprocal relationship (also known as coupling) between actin and nucleotide affinity.
-===Structure of nucleotide-free scallop myosin S1===
+Myosin subfragment 1 structures reveal a partially bound nucleotide and a complex salt bridge that helps couple nucleotide and actin binding.,Risal D, Gourinath S, Himmel DM, Szent-Gyorgyi AG, Cohen C Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8930-5. Epub 2004 Jun 7. PMID:15184651<ref>PMID:15184651</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15184651}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1sr6" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15184651 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_15184651}}
-==About this Structure==
-[[1sr6]] is a 3 chain structure of [[Myosin]] with sequence from [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR6 OCA].
 ==See Also==
-*[[Myosin]]
+*[[Myosin 3D Structures|Myosin 3D Structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:15184651</ref><ref group="xtra">PMID:14656445</ref><ref group="xtra">PMID:12297624</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Argopecten irradians]]
-[[Category: Cohen, C.]]
+[[Category: Large Structures]]
-[[Category: Gourinath, S.]]
+[[Category: Cohen C]]
-[[Category: Himmel, D M.]]
+[[Category: Gourinath S]]
-[[Category: Risal, D.]]
+[[Category: Himmel DM]]
-[[Category: Szent-Gyorgyi, A G.]]
+[[Category: Risal D]]
-[[Category: Complex salt bridge]]
+[[Category: Szent-Gyorgyi AG]]
-[[Category: Contractile protein]]
-[[Category: Near rigor]]
-[[Category: Novel conformation of nucleotide]]
-[[Category: Scallop myosin s1]]