1sh0: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1sh0.jpg|left|200px]]<br /><applet load="1sh0" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1sh0, resolution 2.17&Aring;" />
-'''Crystal Structure of Norwalk Virus Polymerase (Triclinic)'''<br />
-==Overview==
+==Crystal Structure of Norwalk Virus Polymerase (Triclinic)==
-Norwalk virus is a major cause of acute gastroenteritis for which, effective treatments are sorely lacking. To provide a basis for the, rational design of novel antiviral agents, the main replication enzyme in, Norwalk virus, the virally encoded RNA-dependent RNA polymerase (RdRP), has been expressed in an enzymatically active form, and its structure has, been crystallographically determined both in the presence and absence of, divalent metal cations. Although the overall fold of the enzyme is similar, to that seen previously in the RdRP from rabbit hemorrhagic disease virus, the carboxyl terminus, surprisingly, is located in the active site cleft, in five independent copies of the protein in three distinct crystal forms., The location of this carboxyl-terminal segment appears to interfere with, the binding of double-stranded RNA in the active site cleft and may play a, role in the initiation of RNA synthesis or mediate interactions with, accessory replication proteins.
+<StructureSection load='1sh0' size='340' side='right'caption='[[1sh0]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1sh0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SH0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sh0 OCA], [https://pdbe.org/1sh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sh0 RCSB], [https://www.ebi.ac.uk/pdbsum/1sh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sh0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q70ET3_9CALI Q70ET3_9CALI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1sh0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sh0 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Norwalk virus is a major cause of acute gastroenteritis for which effective treatments are sorely lacking. To provide a basis for the rational design of novel antiviral agents, the main replication enzyme in Norwalk virus, the virally encoded RNA-dependent RNA polymerase (RdRP), has been expressed in an enzymatically active form, and its structure has been crystallographically determined both in the presence and absence of divalent metal cations. Although the overall fold of the enzyme is similar to that seen previously in the RdRP from rabbit hemorrhagic disease virus, the carboxyl terminus, surprisingly, is located in the active site cleft in five independent copies of the protein in three distinct crystal forms. The location of this carboxyl-terminal segment appears to interfere with the binding of double-stranded RNA in the active site cleft and may play a role in the initiation of RNA synthesis or mediate interactions with accessory replication proteins.
-==About this Structure==
+Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft.,Ng KK, Pendas-Franco N, Rojo J, Boga JA, Machin A, Alonso JM, Parra F J Biol Chem. 2004 Apr 16;279(16):16638-45. Epub 2004 Feb 5. PMID:14764591<ref>PMID:14764591</ref>
-SH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SH0 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft., Ng KK, Pendas-Franco N, Rojo J, Boga JA, Machin A, Alonso JM, Parra F, J Biol Chem. 2004 Apr 16;279(16):16638-45. Epub 2004 Feb 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14764591 14764591]
+</div>
+<div class="pdbe-citations 1sh0" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Norwalk virus]]
-[[Category: RNA-directed RNA polymerase]]
+[[Category: Alonso JM]]
-[[Category: Single protein]]
+[[Category: Boga JA]]
-[[Category: Alonso, J.M.]]
+[[Category: Machin A]]
-[[Category: Boga, J.A.]]
+[[Category: Ng KK]]
-[[Category: Machin, A.]]
+[[Category: Parra F]]
-[[Category: Ng, K.K.]]
+[[Category: Pendas-Franco N]]
-[[Category: Parra, F.]]
+[[Category: Rojo J]]
-[[Category: Pendas-Franco, N.]]
-[[Category: Rojo, J.]]
-[[Category: rna polymerase]]
-[[Category: viral replication enzyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:28:36 2007''