1sf3: Difference between revisions
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==Structure of the reduced form of the P94A mutant of amicyanin== | ==Structure of the reduced form of the P94A mutant of amicyanin== | ||
<StructureSection load='1sf3' size='340' side='right' caption='[[1sf3]], [[Resolution|resolution]] 1.05Å' scene=''> | <StructureSection load='1sf3' size='340' side='right'caption='[[1sf3]], [[Resolution|resolution]] 1.05Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1sf3]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1sf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SF3 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
< | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sf3 OCA], [https://pdbe.org/1sf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sf3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sf3 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/AMCY_PARDE AMCY_PARDE] Primary acceptor of electrons from methylamine dehydrogenase. Passes those electrons on either a soluble cytochrome c or to pseudoazurin. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</div> | </div> | ||
<div class="pdbe-citations 1sf3" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1sf3" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Amicyanin 3D structures|Amicyanin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Paracoccus denitrificans]] | ||
[[Category: | [[Category: Carrell CJ]] | ||
[[Category: | [[Category: Davidson VL]] | ||
[[Category: | [[Category: Jiang S]] | ||
[[Category: | [[Category: Mathews FS]] | ||
[[Category: | [[Category: Sun D]] | ||
Latest revision as of 09:16, 23 August 2023
Structure of the reduced form of the P94A mutant of amicyaninStructure of the reduced form of the P94A mutant of amicyanin
Structural highlights
FunctionAMCY_PARDE Primary acceptor of electrons from methylamine dehydrogenase. Passes those electrons on either a soluble cytochrome c or to pseudoazurin. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (0.99-1.1 A) structures of both the P94F and P94A amicyanin have been determined in the oxidized and reduced states. In each amicyanin mutant, an electron-withdrawing hydrogen bond to the copper-coordinating thiolate sulfur of Cys92 is introduced by movement of the amide nitrogens of Phe94 and Ala94 much closer to the thiolate sulfur than in wild-type amicyanin. This is the likely explanation for the much more positive E(m) values which result from each of these mutations. The observed decrease in the pK(a) value for the pH dependence of the E(m) value that is seen in the mutants seems to be correlated with steric hindrance to the rotation of the His95 copper ligand which results from the mutations. In wild-type amicyanin the His95 side chain undergoes a redox and pH-dependent conformational change which accounts for the pH dependence of the E(m) value of amicyanin. The reduced P94A amicyanin exhibits two alternate conformations with the positions of the copper 1.4 A apart. In one of these conformations, a water molecule appears to have replaced Met98 as a copper ligand. The relevance of these structures to the electron transfer properties of P94F and P94A amicyanin are also discussed. Structural studies of two mutants of amicyanin from Paracoccus denitrificans that stabilize the reduced state of the copper.,Carrell CJ, Sun D, Jiang S, Davidson VL, Mathews FS Biochemistry. 2004 Jul 27;43(29):9372-80. PMID:15260480[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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