1s03: Difference between revisions

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-{{Seed}}
-[[Image:1s03.png|left|200px]]
-<!--
+==The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex==
-The line below this paragraph, containing "STRUCTURE_1s03", creates the "Structure Box" on the page.
+<StructureSection load='1s03' size='340' side='right'caption='[[1s03]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1s03]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S03 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_1s03|  PDB=1s03  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s03 OCA], [https://pdbe.org/1s03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s03 RCSB], [https://www.ebi.ac.uk/pdbsum/1s03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s03 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RS8_ECOLI RS8_ECOLI] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_01302_B]  Protein S8 is a translational repressor protein, it controls the translation of the spc operon by binding to its mRNA.[HAMAP-Rule:MF_01302_B]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s0/1s03_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s03 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In bacteria, translation of all the ribosomal protein cistrons in the spc operon mRNA is repressed by the binding of the product of one of them, S8, to an internal sequence at the 5' end of the L5 cistron. The way in which the first two genes of the spc operon are regulated, retroregulation, is mechanistically distinct from translational repression by S8 of the genes from L5 onward. A 2.8 A resolution crystal structure has been obtained of Escherichia coli S8 bound to this site. Despite sequence differences, the structure of this complex is almost identical to that of the S8/helix 21 complex seen in the small ribosomal subunit, consistent with the hypothesis that autogenous regulation of ribosomal protein synthesis results from conformational similarities between mRNAs and rRNAs. S8 binding must repress the translation of its own mRNA by inhibiting the formation of a ribosomal initiation complex at the start of the L5 cistron.
-===The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex===
+The structure of a ribosomal protein S8/spc operon mRNA complex.,Merianos HJ, Wang J, Moore PB RNA. 2004 Jun;10(6):954-64. PMID:15146079<ref>PMID:15146079</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1s03" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_15146079}}, adds the Publication Abstract to the page
+*[[Ribosomal protein S8|Ribosomal protein S8]]
-(as it appears on PubMed at http://www.pubmed.gov), where 15146079 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15146079}}
+__TOC__
+</StructureSection>
-==About this Structure==
-S03 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S03 OCA].
-==Reference==
-<ref group="xtra">PMID:15146079</ref><references group="xtra"/>
 [[Category: Escherichia coli]]
-[[Category: Merianos, H J.]]
+[[Category: Large Structures]]
-[[Category: Moore, P B.]]
+[[Category: Merianos HJ]]
-[[Category: Wang, J.]]
+[[Category: Moore PB]]
-[[Category: Protein-rna complex]]
+[[Category: Wang J]]
-[[Category: Ribosomal]]
-[[Category: Spc operon]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:45:23 2009''