1rxm: Difference between revisions

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-[[Image:1rxm.png|left|200px]]
-{{STRUCTURE_1rxm|  PDB=1rxm  |  SCENE=  }}
+==C-terminal region of FEN-1 bound to A. fulgidus PCNA==
+<StructureSection load='1rxm' size='340' side='right'caption='[[1rxm]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1rxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RXM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rxm OCA], [https://pdbe.org/1rxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rxm RCSB], [https://www.ebi.ac.uk/pdbsum/1rxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rxm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PCNA_ARCFU PCNA_ARCFU] Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rx/1rxm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rxm ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
-===C-terminal region of FEN-1 bound to A. fulgidus PCNA===
+Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair.,Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA Cell. 2004 Jan 9;116(1):39-50. PMID:14718165<ref>PMID:14718165</ref>
-{{ABSTRACT_PUBMED_14718165}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1rxm" style="background-color:#fffaf0;"></div>
-[[1rxm]] is a 2 chain structure of [[Proliferating Cell Nuclear Antigen]] with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXM OCA].
 ==See Also==
-*[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]]
+*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:014718165</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Archaeoglobus fulgidus]]
-[[Category: Chapados, B R.]]
+[[Category: Large Structures]]
-[[Category: Han, S.]]
+[[Category: Chapados BR]]
-[[Category: Hosfield, D J.]]
+[[Category: Han S]]
-[[Category: Qiu, J.]]
+[[Category: Hosfield DJ]]
-[[Category: Shen, B.]]
+[[Category: Qiu J]]
-[[Category: Tainer, J A.]]
+[[Category: Shen B]]
-[[Category: Yelent, B.]]
+[[Category: Tainer JA]]
-[[Category: Beta-zipper]]
+[[Category: Yelent B]]
-[[Category: Hydrophobic anchor]]
-[[Category: Processivity factor]]
-[[Category: Replication]]
-[[Category: Sliding clamp]]
-[[Category: Torus]]