1rf2: Difference between revisions

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-[[Image:1rf2.png|left|200px]]
-<!--
+==Cholera Toxin B-Pentamer Complexed With Bivalent Nitrophenol-Galactoside Ligand BV4==
-The line below this paragraph, containing "STRUCTURE_1rf2", creates the "Structure Box" on the page.
+<StructureSection load='1rf2' size='340' side='right'caption='[[1rf2]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1rf2]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RF2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BV4:1,3-BIS-([3-[3-[3-(4-{3-[3-NITRO-5-(GALACTOPYRANOSYLOXY)-BENZOYLAMINO]-PROPYL}-PIPERAZIN-1-YL)-PROPYLAMINO-3,4-DIOXO-CYCLOBUTENYL]-AMINO-PROPOXY-ETHOXY-ETHOXY]-PROPYL-]AMINO-CARBONYLOXY)-2-AMINO-PROPANE'>BV4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-{{STRUCTURE_1rf2|  PDB=1rf2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf2 OCA], [https://pdbe.org/1rf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rf2 RCSB], [https://www.ebi.ac.uk/pdbsum/1rf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rf2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A series of bivalent ligands of varying length were synthesized to inhibit the receptor-binding process of cholera toxin. Competitive surface receptor binding assays showed that significant potency gains relative to the constituent monovalent ligands were achieved independently from the ability of the extended bivalent ligands to span binding sites within the toxin pentamer. Several models that could account for the unexpected improvement in IC(50) values are examined, taking into account crystallographic analysis of each ligand in complex with the toxin pentamer. Evidence is presented that steric blocking at the receptor binding surface may play a role. The results of our study suggest that the use of relatively short, "nonspanning" bivalent ligands, or monovalent ligands of similar topology and bulk may be an effective way of blocking the interaction of multimeric proteins with their cell surface receptors.
-===Cholera Toxin B-Pentamer Complexed With Bivalent Nitrophenol-Galactoside Ligand BV4===
+Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer.,Pickens JC, Mitchell DD, Liu J, Tan X, Zhang Z, Verlinde CL, Hol WG, Fan E Chem Biol. 2004 Sep;11(9):1205-15. PMID:15380181<ref>PMID:15380181</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15380181}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1rf2" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15380181 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_15380181}}
-==About this Structure==
-[[1rf2]] is a 5 chain structure of [[Cholera toxin]] with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF2 OCA].
 ==See Also==
-*[[Cholera toxin]]
+*[[Cholera toxin 3D structures|Cholera toxin 3D structures]]
+*[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:15380181</ref><references group="xtra"/>
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Vibrio cholerae]]
-[[Category: Fan, E.]]
+[[Category: Fan E]]
-[[Category: Hol, W G.]]
+[[Category: Hol WG]]
-[[Category: Liu, J.]]
+[[Category: Liu J]]
-[[Category: Mitchell, D D.]]
+[[Category: Mitchell DD]]
-[[Category: Pickens, J C.]]
+[[Category: Pickens JC]]
-[[Category: Tan, X.]]
+[[Category: Tan X]]
-[[Category: Verlinde, C L.]]
+[[Category: Verlinde CL]]
-[[Category: Zhang, Z.]]
+[[Category: Zhang Z]]
-[[Category: Bivalent]]
-[[Category: Cholera]]
-[[Category: Complex]]
-[[Category: Pentamer]]
-[[Category: Toxin]]