5hue: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5hue is ON HOLD
+==DAHP synthase from Corynebacterium glutamicum in complex with tryptophan==
+<StructureSection load='5hue' size='340' side='right'caption='[[5hue]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5hue]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HUE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hue OCA], [https://pdbe.org/5hue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hue RCSB], [https://www.ebi.ac.uk/pdbsum/5hue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hue ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8NNL5_CORGL Q8NNL5_CORGL]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Corynebacterium glutamicum is widely used for the industrial production of amino acids, nucleotides, and vitamins. The shikimate pathway enzymes DAHP synthase (CgDS; Cg2391) and chorismate mutase (CgCM; Cgl0853) play a key role for the biosynthesis of aromatic compounds. Here we show that CgCM requires the formation of a complex with CgDS to achieve full activity, and that both CgCM and CgDS are feedback regulated by aromatic amino acids binding to CgDS. Kinetic analysis showed that Phe and Tyr inhibit CgCM activity by inter-enzyme allostery, whereas Trp binding to CgDS strongly activates CgCM. Mechanistic insights were gained from crystal structures of the CgCM homodimer, tetrameric CgDS, and the heterooctameric CgCM-CgDS complex, refined to 1.1, 2.5, and 2.2 A resolution, respectively. Structural details from the allosteric binding sites reveal that DAHP synthase is recruited as the dominant regulatory platform to control the shikimate pathway, similar to the corresponding enzyme complex from Mycobacterium tuberculosis.
-Authors: Burschowsky, D., Heim, J.B., Thorbjoernsrud, H.V., Krengel, U.
+Inter-enzyme allosteric regulation of chorismate mutase in Corynebacterium glutamicum: Structural basis of feedback activation by Trp.,Burschowsky D, Thorbjornsrud HV, Heim JB, Fahrig-Kamarauskaite J, Wurth-Roderer K, Kast P, Krengel U Biochemistry. 2017 Nov 27. doi: 10.1021/acs.biochem.7b01018. PMID:29178787<ref>PMID:29178787</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Thorbjoernsrud, H.V]]
+<div class="pdbe-citations 5hue" style="background-color:#fffaf0;"></div>
-[[Category: Heim, J.B]]
-[[Category: Krengel, U]]
+==See Also==
-[[Category: Burschowsky, D]]
+*[[DAHP synthase 3D structures|DAHP synthase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Corynebacterium glutamicum]]
+[[Category: Large Structures]]
+[[Category: Burschowsky D]]
+[[Category: Heim JB]]
+[[Category: Krengel U]]
+[[Category: Thorbjoernsrud HV]]