3k3a: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:3k3a.jpg|left|200px]]
-<!--
+==Crystal Structure of B/Perth Neuraminidase D197E mutant in complex with Oseltamivir==
-The line below this paragraph, containing "STRUCTURE_3k3a", creates the "Structure Box" on the page.
+<StructureSection load='3k3a' size='340' side='right'caption='[[3k3a]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3k3a]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus_(B/Perth/211/2001) Influenza B virus (B/Perth/211/2001)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K3A FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G39:(3R,4R,5S)-4-(ACETYLAMINO)-5-AMINO-3-(PENTAN-3-YLOXY)CYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>G39</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
-{{STRUCTURE_3k3a|  PDB=3k3a  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k3a OCA], [https://pdbe.org/3k3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k3a RCSB], [https://www.ebi.ac.uk/pdbsum/3k3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k3a ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q3S340_9INFB Q3S340_9INFB]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have identified a virus, B/Perth/211/2001, with a spontaneous mutation, D197E in the neuraminidase (NA), which confers cross-resistance to all NA inhibitors. We analyzed enzyme properties of the D197 and E197 NAs and compared these to a D197N NA, known to arise after oseltamivir treatment. Zanamivir and peramivir bound slowly to the wild type NA, but binding of oseltamivir was more rapid. The D197E/N mutations resulted in faster binding of all three inhibitors. Analysis of the crystal structures of D197 and E197 NAs with and without inhibitors showed that the D197E mutation compromised the interaction of neighboring R150 with the N-acetyl group, common to the substrate sialic acid and all NA inhibitors. Although rotation of the E275 in the NA active site occurs upon binding peramivir in both the D197 and E197 NAs, this does not occur upon binding oseltamivir in the E197 NA. Lack of the E275 rotation would also account for the loss of slow binding and the partial resistance of influenza B wild type NAs to oseltamivir.
-===Crystal Structure of B/Perth Neruaminidase D197E mutant in complex with Oseltamivir===
+Structural and Functional Basis of Resistance to Neuraminidase Inhibitors of Influenza B Viruses.,Oakley AJ, Barrett S, Peat TS, Newman J, Streltsov VA, Waddington L, Saito T, Tashiro M, McKimm-Breschkin JL J Med Chem. 2010 Aug 9. PMID:20695427<ref>PMID:20695427</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3k3a" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20695427}}, adds the Publication Abstract to the page
+*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20695427 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20695427}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-K3A is a 16 chains structure with sequences from [http://en.wikipedia.org/wiki/Influenza_b_virus Influenza b virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3A OCA].
+[[Category: McKimm-Breschkin JL]]
+[[Category: Oakley AJ]]
-==Reference==
-<ref group="xtra">PMID:20695427</ref><references group="xtra"/>
-[[Category: Exo-alpha-sialidase]]
-[[Category: Influenza b virus]]
-[[Category: McKimm-Breschkin, J L.]]
-[[Category: Oakley, A J.]]
-[[Category: 196618-13-0]]
-[[Category: Cell membrane]]
-[[Category: Glycosidase]]
-[[Category: Gs-4071]]
-[[Category: Hydrolase]]
-[[Category: Influenza]]
-[[Category: Membrane]]
-[[Category: Mutation]]
-[[Category: Neuraminidase]]
-[[Category: Oseltamivir]]
-[[Category: Resistance]]
-[[Category: Tamiflu]]
-[[Category: Transmembrane]]
-[[Category: Virion]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep  1 09:48:13 2010''