2drz: Difference between revisions

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-{{Seed}}
-[[Image:2drz.png|left|200px]]
-<!--
+==Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose==
-The line below this paragraph, containing "STRUCTURE_2drz", creates the "Structure Box" on the page.
+<StructureSection load='2drz' size='340' side='right'caption='[[2drz]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2drz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DRZ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2drz|  PDB=2drz  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2drz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2drz OCA], [https://pdbe.org/2drz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2drz RCSB], [https://www.ebi.ac.uk/pdbsum/2drz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2drz ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O96048_LUMTE O96048_LUMTE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
-===Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose===
+Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry.,Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683<ref>PMID:17234683</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2drz" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17234683}}, adds the Publication Abstract to the page
+*[[Galactose-binding lectin|Galactose-binding lectin]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17234683 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17234683}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-DRZ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRZ OCA].
-==Reference==
-<ref group="xtra">PMID:17234683</ref><ref group="xtra">PMID:15388944</ref><ref group="xtra">PMID:9603958</ref><references group="xtra"/>
 [[Category: Lumbricus terrestris]]
-[[Category: Fujimoto, Z.]]
+[[Category: Fujimoto Z]]
-[[Category: Suzuki, R.]]
+[[Category: Suzuki R]]
-[[Category: Beta-trefoil fold]]
-[[Category: Earthworm lumbricus terrestri]]
-[[Category: Galactose]]
-[[Category: In vitro evolution]]
-[[Category: Sialic acid]]
-[[Category: Sugar binding protein]]
-[[Category: Sugar complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug  5 08:52:20 2009''