1w09: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1w09.png|left|200px]]
-{{STRUCTURE_1w09|  PDB=1w09  |  SCENE=  }}
+==Solution structure of the cis form of the human alpha-hemoglobin stabilizing protein (AHSP)==
+<StructureSection load='1w09' size='340' side='right'caption='[[1w09]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1w09]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W09 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w09 OCA], [https://pdbe.org/1w09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w09 RCSB], [https://www.ebi.ac.uk/pdbsum/1w09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w09 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AHSP_HUMAN AHSP_HUMAN] Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.<ref>PMID:12066189</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structure of alpha-hemoglobin stabilizing protein (AHSP), a molecular chaperone for free alpha-hemoglobin, has been determined using NMR spectroscopy. The protein native state shows conformational heterogeneity attributable to the isomerization of the peptide bond preceding a conserved proline residue. The two equally populated cis and trans forms both adopt an elongated antiparallel three alpha-helix bundle fold but display major differences in the loop between the first two helices and at the C terminus of helix 3. Proline to alanine single point mutation of the residue Pro-30 prevents the cis/trans isomerization. The structure of the P30A mutant is similar to the structure of the trans form of AHSP in the loop 1 region. Both the wild-type AHSP and the P30A mutant bind to alpha-hemoglobin, and the wild-type conformational heterogeneity is quenched upon complex formation, suggesting that just one conformation is the active form. Changes in chemical shift observed upon complex formation identify a binding interface comprising the C terminus of helix 1, the loop 1, and the N terminus of helix 2, with the exposed residues Phe-47 and Tyr-51 being attractive targets for molecular recognition. The characteristics of this interface suggest that AHSP binds at the intradimer alpha1beta1 interface in tetrameric HbA.
-===SOLUTION STRUCTURE OF THE CIS FORM OF THE HUMAN ALPHA-HEMOGLOBIN STABILIZING PROTEIN (AHSP)===
+NMR structure of the alpha-hemoglobin stabilizing protein: insights into conformational heterogeneity and binding.,Santiveri CM, Perez-Canadillas JM, Vadivelu MK, Allen MD, Rutherford TJ, Watkins NA, Bycroft M J Biol Chem. 2004 Aug 13;279(33):34963-70. Epub 2004 Jun 3. PMID:15178680<ref>PMID:15178680</ref>
-{{ABSTRACT_PUBMED_15178680}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1w09" style="background-color:#fffaf0;"></div>
-[[1w09]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W09 OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:015178680</ref><references group="xtra"/>
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Allen, M D.]]
+[[Category: Large Structures]]
-[[Category: Bycroft, M.]]
+[[Category: Allen MD]]
-[[Category: Perez-Canadillas, J M.]]
+[[Category: Bycroft M]]
-[[Category: Rutherford, T J.]]
+[[Category: Perez-Canadillas JM]]
-[[Category: Santiveri, C M.]]
+[[Category: Rutherford TJ]]
-[[Category: Vadivelu, M K.]]
+[[Category: Santiveri CM]]
-[[Category: Watkins, N A.]]
+[[Category: Vadivelu MK]]
-[[Category: Ahsp nmr structure]]
+[[Category: Watkins NA]]
-[[Category: Alpha-hemoglobin binding]]
-[[Category: Alpha-thalassaemia]]
-[[Category: Chaperone]]
-[[Category: Proline cis/trans isomerization]]