6pwv: Difference between revisions

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-'''Unreleased structure'''
-The entry 6pwv is ON HOLD
+==Cryo-EM structure of MLL1 core complex bound to the nucleosome==
+<SX load='6pwv' size='340' side='right' viewer='molstar' caption='[[6pwv]], [[Resolution|resolution]] 6.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6pwv]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PWV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwv OCA], [https://pdbe.org/6pwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pwv RCSB], [https://www.ebi.ac.uk/pdbsum/6pwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwv ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBBP5_HUMAN RBBP5_HUMAN] In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.<ref>PMID:19556245</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mixed lineage leukemia (MLL) family histone methyltransferases are enzymes that deposit histone H3 Lys4 (K4) mono-/di-/tri-methylation and regulate gene expression in mammals. Despite extensive structural and biochemical studies, the molecular mechanisms whereby the MLL complexes recognize histone H3K4 within nucleosome core particles (NCPs) remain unclear. Here we report the single-particle cryo-electron microscopy (cryo-EM) structure of the NCP-bound human MLL1 core complex. We show that the MLL1 core complex anchors to the NCP via the conserved RbBP5 and ASH2L, which interact extensively with nucleosomal DNA and the surface close to the N-terminal tail of histone H4. Concurrent interactions of RbBP5 and ASH2L with the NCP uniquely align the catalytic MLL1(SET) domain at the nucleosome dyad, thereby facilitating symmetrical access to both H3K4 substrates within the NCP. Our study sheds light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.
-Authors:
+Cryo-EM structure of the human MLL1 core complex bound to the nucleosome.,Park SH, Ayoub A, Lee YT, Xu J, Kim H, Zheng W, Zhang B, Sha L, An S, Zhang Y, Cianfrocco MA, Su M, Dou Y, Cho US Nat Commun. 2019 Dec 5;10(1):5540. doi: 10.1038/s41467-019-13550-2. PMID:31804488<ref>PMID:31804488</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6pwv" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Histone 3D structures|Histone 3D structures]]
+*[[Histone methyltransferase 3D structures|Histone methyltransferase 3D structures]]
+*[[Retinoblastoma-binding protein 3D structures|Retinoblastoma-binding protein 3D structures]]
+*[[WD-repeat protein 3D structures|WD-repeat protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Xenopus laevis]]
+[[Category: Ayoub A]]
+[[Category: Cho U]]
+[[Category: Cianfrocco MA]]
+[[Category: Dou Y]]
+[[Category: Lee YT]]
+[[Category: Park SH]]
+[[Category: Su M]]
+[[Category: Xu J]]
+[[Category: Zhang B]]
+[[Category: Zhang W]]
+[[Category: Zhang Y]]