4ow3: Difference between revisions

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==Thermolysin structure determined by free-electron laser==
==Thermolysin structure determined by free-electron laser==
<StructureSection load='4ow3' size='340' side='right' caption='[[4ow3]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='4ow3' size='340' side='right'caption='[[4ow3]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ow3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OW3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ow3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OW3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ow3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ow3 OCA], [http://pdbe.org/4ow3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ow3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ow3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ow3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ow3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ow3 OCA], [https://pdbe.org/4ow3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ow3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ow3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ow3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.  
[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 21: Line 21:


==See Also==
==See Also==
*[[Thermolysin|Thermolysin]]
*[[Thermolysin 3D structures|Thermolysin 3D structures]]
== References ==
== References ==
<references/>
<references/>
Line 27: Line 27:
</StructureSection>
</StructureSection>
[[Category: Bacillus thermoproteolyticus]]
[[Category: Bacillus thermoproteolyticus]]
[[Category: Thermolysin]]
[[Category: Large Structures]]
[[Category: Adams, P D]]
[[Category: Adams PD]]
[[Category: Alonso-Mori, R]]
[[Category: Alonso-Mori R]]
[[Category: Bergmann, U]]
[[Category: Bergmann U]]
[[Category: Bogan, M J]]
[[Category: Bogan MJ]]
[[Category: Boutet, S]]
[[Category: Boutet S]]
[[Category: Brewster, A S]]
[[Category: Brewster AS]]
[[Category: DiFiore, D]]
[[Category: DiFiore D]]
[[Category: Echols, N]]
[[Category: Echols N]]
[[Category: Fry, A R]]
[[Category: Fry AR]]
[[Category: Gildea, R J]]
[[Category: Gildea RJ]]
[[Category: Glatzel, P]]
[[Category: Glatzel P]]
[[Category: Glockner, C]]
[[Category: Glockner C]]
[[Category: Grosse-Kunstleve, R W]]
[[Category: Grosse-Kunstleve RW]]
[[Category: Gul, S]]
[[Category: Gul S]]
[[Category: Han, G]]
[[Category: Han G]]
[[Category: Hattne, J]]
[[Category: Hattne J]]
[[Category: Hellmich, J]]
[[Category: Hellmich J]]
[[Category: Kern, J]]
[[Category: Kern J]]
[[Category: Koglin, J E]]
[[Category: Koglin JE]]
[[Category: Laksmono, H]]
[[Category: Laksmono H]]
[[Category: Lampe, A]]
[[Category: Lampe A]]
[[Category: Lassalle-Kaiser, B]]
[[Category: Lassalle-Kaiser B]]
[[Category: Latimer, M J]]
[[Category: Latimer MJ]]
[[Category: Messerschmidt, M]]
[[Category: Messerschmidt M]]
[[Category: Messinger, J]]
[[Category: Messinger J]]
[[Category: Miahnahri, A]]
[[Category: Miahnahri A]]
[[Category: Milathianaki, D]]
[[Category: Milathianaki D]]
[[Category: Sauter, N K]]
[[Category: Sauter NK]]
[[Category: Schafer, D W]]
[[Category: Schafer DW]]
[[Category: Seibert, M M]]
[[Category: Seibert MM]]
[[Category: Sellberg, J]]
[[Category: Sellberg J]]
[[Category: Sierra, R G]]
[[Category: Sierra RG]]
[[Category: Sokaras, D]]
[[Category: Sokaras D]]
[[Category: Tran, R]]
[[Category: Tran R]]
[[Category: Weng, T C]]
[[Category: Weng T-C]]
[[Category: White, W E]]
[[Category: White WE]]
[[Category: Williams, G J]]
[[Category: Williams GJ]]
[[Category: Yachandra, V K]]
[[Category: Yachandra VK]]
[[Category: Yano, J]]
[[Category: Yano J]]
[[Category: Zouni, A]]
[[Category: Zouni A]]
[[Category: Zwart, P H]]
[[Category: Zwart PH]]
[[Category: Hydrolase]]
[[Category: Protease]]

Latest revision as of 13:06, 16 August 2023

Thermolysin structure determined by free-electron laserThermolysin structure determined by free-electron laser

Structural highlights

4ow3 is a 1 chain structure with sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THER_BACTH Extracellular zinc metalloprotease.

Publication Abstract from PubMed

X-ray free-electron laser (XFEL) sources enable the use of crystallography to solve three-dimensional macromolecular structures under native conditions and without radiation damage. Results to date, however, have been limited by the challenge of deriving accurate Bragg intensities from a heterogeneous population of microcrystals, while at the same time modeling the X-ray spectrum and detector geometry. Here we present a computational approach designed to extract meaningful high-resolution signals from fewer diffraction measurements.

Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.,Hattne J, Echols N, Tran R, Kern J, Gildea RJ, Brewster AS, Alonso-Mori R, Glockner C, Hellmich J, Laksmono H, Sierra RG, Lassalle-Kaiser B, Lampe A, Han G, Gul S, DiFiore D, Milathianaki D, Fry AR, Miahnahri A, White WE, Schafer DW, Seibert MM, Koglin JE, Sokaras D, Weng TC, Sellberg J, Latimer MJ, Glatzel P, Zwart PH, Grosse-Kunstleve RW, Bogan MJ, Messerschmidt M, Williams GJ, Boutet S, Messinger J, Zouni A, Yano J, Bergmann U, Yachandra VK, Adams PD, Sauter NK Nat Methods. 2014 May;11(5):545-8. doi: 10.1038/nmeth.2887. Epub 2014 Mar 16. PMID:24633409[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Hattne J, Echols N, Tran R, Kern J, Gildea RJ, Brewster AS, Alonso-Mori R, Glockner C, Hellmich J, Laksmono H, Sierra RG, Lassalle-Kaiser B, Lampe A, Han G, Gul S, DiFiore D, Milathianaki D, Fry AR, Miahnahri A, White WE, Schafer DW, Seibert MM, Koglin JE, Sokaras D, Weng TC, Sellberg J, Latimer MJ, Glatzel P, Zwart PH, Grosse-Kunstleve RW, Bogan MJ, Messerschmidt M, Williams GJ, Boutet S, Messinger J, Zouni A, Yano J, Bergmann U, Yachandra VK, Adams PD, Sauter NK. Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers. Nat Methods. 2014 May;11(5):545-8. doi: 10.1038/nmeth.2887. Epub 2014 Mar 16. PMID:24633409 doi:http://dx.doi.org/10.1038/nmeth.2887

4ow3, resolution 2.10Å

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