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[[Image:1lc4.gif|left|200px]]


{{Structure
==Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site==
|PDB= 1lc4 |SIZE=350|CAPTION= <scene name='initialview01'>1lc4</scene>, resolution 2.54&Aring;
<StructureSection load='1lc4' size='340' side='right'caption='[[1lc4]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
<table><tr><td colspan='2'>[[1lc4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LC4 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.54&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc4 OCA], [https://pdbe.org/1lc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lc4 RCSB], [https://www.ebi.ac.uk/pdbsum/1lc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lc4 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1j7t|1J7T]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc4 OCA], [http://www.ebi.ac.uk/pdbsum/1lc4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lc4 RCSB]</span>
<div style="background-color:#fffaf0;">
}}
== Publication Abstract from PubMed ==
 
'''Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site'''
 
 
==Overview==
Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient.
Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient.


==About this Structure==
Crystal structure of a complex between the aminoglycoside tobramycin and an oligonucleotide containing the ribosomal decoding a site.,Vicens Q, Westhof E Chem Biol. 2002 Jun;9(6):747-55. PMID:12079787<ref>PMID:12079787</ref>
1LC4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC4 OCA].
 
==Reference==
Crystal structure of a complex between the aminoglycoside tobramycin and an oligonucleotide containing the ribosomal decoding a site., Vicens Q, Westhof E, Chem Biol. 2002 Jun;9(6):747-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079787 12079787]
[[Category: Protein complex]]
[[Category: Vicens, Q.]]
[[Category: Westhof, E.]]
[[Category: adenine bulge]]
[[Category: antibiotic-rna complex]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:00:57 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1lc4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vicens Q]]
[[Category: Westhof E]]

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