1l4a: Difference between revisions

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-[[Image:1l4a.gif|left|200px]]<br /><applet load="1l4a" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1l4a, resolution 2.95&Aring;" />
-'''X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI'''<br />
-==Overview==
+==X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI==
-Nerve terminals release neurotransmitters from vesicles into the synaptic, cleft upon transient increases in intracellular Ca(2+). This exocytotic, process requires the formation of trans SNARE complexes and is regulated, by accessory proteins including the complexins. Here we report the crystal, structure of a squid core complexin-SNARE complex at 2.95-A resolution. A, helical segment of complexin binds in anti-parallel fashion to the, four-helix bundle of the core SNARE complex and interacts at its C, terminus with syntaxin and synaptobrevin around the ionic zero layer of, the SNARE complex. We propose that this structure is part of a, multiprotein fusion machinery that regulates vesicle fusion at a late, pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by, acting directly or indirectly on complexin, thus allowing the, conformational transitions of the trans SNARE complex that are thought to, drive membrane fusion.
+<StructureSection load='1l4a' size='340' side='right'caption='[[1l4a]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1l4a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L4A FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l4a OCA], [https://pdbe.org/1l4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l4a RCSB], [https://www.ebi.ac.uk/pdbsum/1l4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l4a ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYB_DORPE SYB_DORPE] Intrinsic membrane protein of small synaptic vesicles.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l4/1l4a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l4a ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Nerve terminals release neurotransmitters from vesicles into the synaptic cleft upon transient increases in intracellular Ca(2+). This exocytotic process requires the formation of trans SNARE complexes and is regulated by accessory proteins including the complexins. Here we report the crystal structure of a squid core complexin-SNARE complex at 2.95-A resolution. A helical segment of complexin binds in anti-parallel fashion to the four-helix bundle of the core SNARE complex and interacts at its C terminus with syntaxin and synaptobrevin around the ionic zero layer of the SNARE complex. We propose that this structure is part of a multiprotein fusion machinery that regulates vesicle fusion at a late pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by acting directly or indirectly on complexin, thus allowing the conformational transitions of the trans SNARE complex that are thought to drive membrane fusion.
-==About this Structure==
+X-ray structure of a neuronal complexin-SNARE complex from squid.,Bracher A, Kadlec J, Betz H, Weissenhorn W J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:12004067<ref>PMID:12004067</ref>
-L4A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Loligo_plei Loligo plei]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L4A OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-X-ray structure of a neuronal complexin-SNARE complex from squid., Bracher A, Kadlec J, Betz H, Weissenhorn W, J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12004067 12004067]
+</div>
-[[Category: Loligo plei]]
+<div class="pdbe-citations 1l4a" style="background-color:#fffaf0;"></div>
-[[Category: Protein complex]]
-[[Category: Betz, H.]]
-[[Category: Bracher, A.]]
-[[Category: Kadlec, J.]]
-[[Category: Weissenhorn, W.]]
-[[Category: membrane fusion]]
-[[Category: neurotransmission]]
-[[Category: snare]]
-[[Category: snare complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:14:46 2007''
+==See Also==
+*[[Syntaxin 3D structures|Syntaxin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Doryteuthis pealeii]]
+[[Category: Large Structures]]
+[[Category: Betz H]]
+[[Category: Bracher A]]
+[[Category: Kadlec J]]
+[[Category: Weissenhorn W]]