1kil: Difference between revisions

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-[[Image:1kil.gif|left|200px]]
- {{Structure
+==Three-dimensional structure of the complexin/SNARE complex==
-|PDB= 1kil |SIZE=350|CAPTION= <scene name='initialview01'>1kil</scene>, resolution 2.30&Aring;
+<StructureSection load='1kil' size='340' side='right'caption='[[1kil]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
+<table><tr><td colspan='2'>[[1kil]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The November 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIL FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE= Synaptobrevin 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Syntaxin 1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), SNAP-25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Complexin 1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kil OCA], [https://pdbe.org/1kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kil RCSB], [https://www.ebi.ac.uk/pdbsum/1kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kil ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kil_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kil ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release.
-'''Three-dimensional structure of the complexin/SNARE complex'''
+Three-dimensional structure of the complexin/SNARE complex.,Chen X, Tomchick DR, Kovrigin E, Arac D, Machius M, Sudhof TC, Rizo J Neuron. 2002 Jan 31;33(3):397-409. PMID:11832227<ref>PMID:11832227</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1kil" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release.
+*[[Complexin|Complexin]]
+*[[Synaptosomal-associated protein|Synaptosomal-associated protein]]
-==About this Structure==
+*[[Syntaxin 3D structures|Syntaxin 3D structures]]
-KIL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIL OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-Three-dimensional structure of the complexin/SNARE complex., Chen X, Tomchick DR, Kovrigin E, Arac D, Machius M, Sudhof TC, Rizo J, Neuron. 2002 Jan 31;33(3):397-409. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11832227 11832227]
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
+[[Category: RCSB PDB Molecule of the Month]]
 [[Category: Rattus norvegicus]]
-[[Category: Arac, D.]]
+[[Category: SNARE Proteins]]
-[[Category: Chen, X.]]
+[[Category: Arac D]]
-[[Category: Kovrigin, E.]]
+[[Category: Chen X]]
-[[Category: Machius, M.]]
+[[Category: Kovrigin E]]
-[[Category: Rizo, J.]]
+[[Category: Machius M]]
-[[Category: Sudhof, T C.]]
+[[Category: Rizo J]]
-[[Category: Tomchick, D.]]
+[[Category: Sudhof TC]]
-[[Category: MG]]
+[[Category: Tomchick D]]
-[[Category: helix bound to four helix bundle]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:56 2008''