5h3v: Difference between revisions
New page: '''Unreleased structure''' The entry 5h3v is ON HOLD Authors: Jin, Z., Wu, Y., Zhao, Y., Sun, L., Keegan, R.M., Liu, Y., Isupov, M.N. Description: Crystal structure of a Type IV Secret... |
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==Crystal structure of a Type IV Secretion System Component CagX in Helicobacter pylori== | |||
<StructureSection load='5h3v' size='340' side='right'caption='[[5h3v]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5h3v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H3V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3v OCA], [https://pdbe.org/5h3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h3v RCSB], [https://www.ebi.ac.uk/pdbsum/5h3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3v ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O25263_HELPY O25263_HELPY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Helicobacter pylori, a Gram-negative bacterial pathogen prevalent in the human population, is the causative agent of severe gastric diseases. An H. pylori type IV secretion (T4S) system encoded by the cytotoxin-associated gene pathogenicity island (cagPAI) is responsible for communication with host cells. As a component of the cagPAI T4S system core complex, CagX plays an important role in virulence-protein translocation into the host cells. In this work, the crystal structure of the C-terminal domain of CagX (CagXct), which is a homologue of the VirB9 protein from the VirB/D4 T4S system, is presented. CagXct is only the second three-dimensional structure to be elucidated of a VirB9-like protein. Another homologue, TraO, which is encoded on the Escherichia coli conjugative plasmid pKM101, shares only 19% sequence identity with CagXct; however, there is a remarkable similarity in tertiary structure between these two beta-sandwich protein domains. Most of the residues that are conserved between CagXct and TraO are located within the protein core and appear to be responsible for the preservation of this domain fold. The studies presented here will contribute to our understanding of different bacterial T4S systems. | |||
Crystal structure of the type IV secretion system component CagX from Helicobacter pylori.,Zhang J, Fan F, Zhao Y, Sun L, Liu Y, Keegan RM, Isupov MN, Wu Y Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):167-173. doi:, 10.1107/S2053230X17001376. Epub 2017 Feb 28. PMID:28291753<ref>PMID:28291753</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5h3v" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Helicobacter pylori]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Isupov MN]] | |||
[[Category: Keegan RM]] | |||
[[Category: Liu Y]] | |||
[[Category: Sun L]] | |||
[[Category: Wu Y]] | |||
[[Category: Zhang J]] | |||
[[Category: Zhao Y]] | |||
Latest revision as of 10:22, 9 August 2023
Crystal structure of a Type IV Secretion System Component CagX in Helicobacter pyloriCrystal structure of a Type IV Secretion System Component CagX in Helicobacter pylori
Structural highlights
FunctionPublication Abstract from PubMedHelicobacter pylori, a Gram-negative bacterial pathogen prevalent in the human population, is the causative agent of severe gastric diseases. An H. pylori type IV secretion (T4S) system encoded by the cytotoxin-associated gene pathogenicity island (cagPAI) is responsible for communication with host cells. As a component of the cagPAI T4S system core complex, CagX plays an important role in virulence-protein translocation into the host cells. In this work, the crystal structure of the C-terminal domain of CagX (CagXct), which is a homologue of the VirB9 protein from the VirB/D4 T4S system, is presented. CagXct is only the second three-dimensional structure to be elucidated of a VirB9-like protein. Another homologue, TraO, which is encoded on the Escherichia coli conjugative plasmid pKM101, shares only 19% sequence identity with CagXct; however, there is a remarkable similarity in tertiary structure between these two beta-sandwich protein domains. Most of the residues that are conserved between CagXct and TraO are located within the protein core and appear to be responsible for the preservation of this domain fold. The studies presented here will contribute to our understanding of different bacterial T4S systems. Crystal structure of the type IV secretion system component CagX from Helicobacter pylori.,Zhang J, Fan F, Zhao Y, Sun L, Liu Y, Keegan RM, Isupov MN, Wu Y Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):167-173. doi:, 10.1107/S2053230X17001376. Epub 2017 Feb 28. PMID:28291753[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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