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[[Image:2plc.jpg|left|200px]]<br /><applet load="2plc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2plc, resolution 2.0&Aring;" />
'''PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES'''<br />


==Overview==
==PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES==
The X-ray crystal structure of the phosphatidylinositol-specific, phospholipase C (PI-PLC) from the human pathogen Listeria monocytogenes, has been determined both in free form at 2.0 A resolution, and in complex, with the competitive inhibitor myo-inositol at 2.6 A resolution. The, structure was solved by a combination of molecular replacement using the, structure of Bacillus cereus PI-PLC and single isomorphous replacement., The enzyme consists of a single (beta alpha)8-barrel domain with the, active site located at the C-terminal side of the beta-barrel. Unlike, other (beta alpha)8-barrels, the barrel in PI-PLC is open because it lacks, hydrogen bonding interactions between beta-strands V and VI. myo-Inositol, binds to the active site pocket by making specific hydrogen bonding, interactions with a number of charged amino acid side-chains as well as a, coplanar stacking interaction with a tyrosine residue. Despite a, relatively low sequence identity of approximately 24%, the structure is, highly homologous to that of B.cereus PI-PLC with an r.m.s. deviation for, 228 common C alpha positions of 1.46 A. Larger differences are found for, loop regions that accommodate most of the numerous amino acid insertions, and deletions. The active site pocket is also well conserved with only two, amino acid replacements directly implicated in inositol binding.
<StructureSection load='2plc' size='340' side='right'caption='[[2plc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2plc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PLC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2plc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2plc OCA], [https://pdbe.org/2plc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2plc RCSB], [https://www.ebi.ac.uk/pdbsum/2plc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2plc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PLC_LISMO PLC_LISMO] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/2plc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2plc ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray crystal structure of the phosphatidylinositol-specific phospholipase C (PI-PLC) from the human pathogen Listeria monocytogenes has been determined both in free form at 2.0 A resolution, and in complex with the competitive inhibitor myo-inositol at 2.6 A resolution. The structure was solved by a combination of molecular replacement using the structure of Bacillus cereus PI-PLC and single isomorphous replacement. The enzyme consists of a single (beta alpha)8-barrel domain with the active site located at the C-terminal side of the beta-barrel. Unlike other (beta alpha)8-barrels, the barrel in PI-PLC is open because it lacks hydrogen bonding interactions between beta-strands V and VI. myo-Inositol binds to the active site pocket by making specific hydrogen bonding interactions with a number of charged amino acid side-chains as well as a coplanar stacking interaction with a tyrosine residue. Despite a relatively low sequence identity of approximately 24%, the structure is highly homologous to that of B.cereus PI-PLC with an r.m.s. deviation for 228 common C alpha positions of 1.46 A. Larger differences are found for loop regions that accommodate most of the numerous amino acid insertions and deletions. The active site pocket is also well conserved with only two amino acid replacements directly implicated in inositol binding.


==About this Structure==
Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes.,Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW J Mol Biol. 1997 Oct 17;273(1):269-82. PMID:9367761<ref>PMID:9367761</ref>
2PLC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_4.6.1.13 Transferred entry: 4.6.1.13], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.10 3.1.4.10] Known structural/functional Site: <scene name='pdbsite=CIC:Inositol+Binding+Site'>CIC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLC OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes., Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW, J Mol Biol. 1997 Oct 17;273(1):269-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9367761 9367761]
</div>
<div class="pdbe-citations 2plc" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Phospholipase C|Phospholipase C]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Listeria monocytogenes]]
[[Category: Listeria monocytogenes]]
[[Category: Single protein]]
[[Category: Heinz DW]]
[[Category: Transferred entry: 4.6.1.13]]
[[Category: Moser J]]
[[Category: Heinz, D.W.]]
[[Category: Moser, J.]]
[[Category: hydrolase]]
[[Category: phospholipid degradation]]
[[Category: virulence factor of human pathogen]]
 
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