2ayq: Difference between revisions

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-{{Seed}}
-[[Image:2ayq.png|left|200px]]
-<!--
+==3-ISOPROPYLMALATE DEHYDROGENASE FROM THE MODERATE FACULTATIVE THERMOPHILE, BACILLUS COAGULANS==
-The line below this paragraph, containing "STRUCTURE_2ayq", creates the "Structure Box" on the page.
+<StructureSection load='2ayq' size='340' side='right'caption='[[2ayq]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ayq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Weizmannia_coagulans Weizmannia coagulans]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ayq 1ayq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYQ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayq OCA], [https://pdbe.org/2ayq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayq RCSB], [https://www.ebi.ac.uk/pdbsum/2ayq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayq ProSAT]</span></td></tr>
-{{STRUCTURE_2ayq|  PDB=2ayq  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LEU3_WEICA LEU3_WEICA] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/2ayq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ayq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile Bacillus coagulans (BcIPMDH) has been determined by the X-ray method. BcIPMDH is a dimeric enzyme composed of two identical subunits, each of which takes an open alpha/beta structure with 11 alpha-helices and 14 beta-strands. The polypeptide is folded into two domains. The first domain is composed of residues 1-101 and 257-356, and the second domain, of residues 102-256. The latter domains of the two subunits are associated with one another by a dyad axis to make the dimer, locally forming a beta-sheet and a four-helix bundle. As compared with the structure of the enzyme from the extreme thermophile Thermus thermophilus (TtIPMDH), a new short beta-sheet (residues 329-330 and 340-341) absent in TtIPMDH is formed by the insertion of 5 residues in BcIPMDH. In terms of determinants for thermostabilization, both consistent and inconsistent changes were found between the two enzymes. The regions including inconsistent changes are formed by different usages of the determinants for stabilizing the loops at different levels. Those in BcIPMDH contain some structural redundancies in length of amino acid sequence and flexibility of residues, which seem to be unnecessary for the enzymatic reaction. Such redundancies are also found in the primary structure of the enzyme of the mesophile Bacillus subtilis, but these parts are more stabilized in BcIPMDH by hydrogen bonds and salt bridges. On the other hand, TtIPMDH is stabilized by reducing such redundant parts. This contrast suggests that different strategies may be preferred for thermostabilization, depending on temperature.
-===3-ISOPROPYLMALATE DEHYDROGENASE FROM THE MODERATE FACULTATIVE THERMOPHILE, BACILLUS COAGULANS===
+Crystal structure of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile, Bacillus coagulans: two strategies for thermostabilization of protein structures.,Tsuchiya D, Sekiguchi T, Takenaka A J Biochem. 1997 Dec;122(6):1092-104. PMID:9498551<ref>PMID:9498551</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ayq" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_9498551}}, adds the Publication Abstract to the page
+*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 9498551 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9498551}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-AYQ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_coagulans Bacillus coagulans]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ayq 1ayq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYQ OCA].
+[[Category: Weizmannia coagulans]]
+[[Category: Takenaka A]]
-==Reference==
+[[Category: Tsuchiya D]]
-<ref group="xtra">PMID:9498551</ref><references group="xtra"/>
-[[Category: 3-isopropylmalate dehydrogenase]]
-[[Category: Bacillus coagulans]]
-[[Category: Takenaka, A.]]
-[[Category: Tsuchiya, D.]]
-[[Category: Leucine biosynthesis]]
-[[Category: Moderate thermophile]]
-[[Category: Oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:26:50 2009''