1vif: Difference between revisions

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-[[Image:1vif.jpg|left|200px]]
-<!--
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-{{STRUCTURE_1vif|  PDB=1vif  |  SCENE=  }}
-'''STRUCTURE OF DIHYDROFOLATE REDUCTASE'''
+==STRUCTURE OF DIHYDROFOLATE REDUCTASE==
+<StructureSection load='1vif' size='340' side='right'caption='[[1vif]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1vif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VIF FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FOL:FOLIC+ACID'>FOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vif OCA], [https://pdbe.org/1vif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vif RCSB], [https://www.ebi.ac.uk/pdbsum/1vif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vif ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric form. The structure of native R67 DHFR has now been solved at 1.7 A resolution and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an unusual pore, 25 A in length, passing through the middle of the molecule. Two folate molecules bind asymmetrically within the pore indicating that the enzyme's active site consists of symmetry related binding surfaces from all four identical units.
-==Overview==
+A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site.,Narayana N, Matthews DA, Howell EE, Nguyen-huu X Nat Struct Biol. 1995 Nov;2(11):1018-25. PMID:7583655<ref>PMID:7583655</ref>
-Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric form. The structure of native R67 DHFR has now been solved at 1.7 A resolution and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an unusual pore, 25 A in length, passing through the middle of the molecule. Two folate molecules bind asymmetrically within the pore indicating that the enzyme's active site consists of symmetry related binding surfaces from all four identical units.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-VIF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VIF OCA].
+</div>
+<div class="pdbe-citations 1vif" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site., Narayana N, Matthews DA, Howell EE, Nguyen-huu X, Nat Struct Biol. 1995 Nov;2(11):1018-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7583655 7583655]
+*[[Dihydrofolate reductase 3D structures|Dihydrofolate reductase 3D structures]]
-[[Category: Dihydrofolate reductase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Howell, E E.]]
+[[Category: Howell EE]]
-[[Category: Matthews, D A.]]
+[[Category: Matthews DA]]
-[[Category: Narayana, N.]]
+[[Category: Narayana N]]
-[[Category: Xuong, N H.]]
+[[Category: Xuong N-H]]
-[[Category: Nadp]]
-[[Category: One-carbon metabolism]]
-[[Category: Oxidoreductase]]
-[[Category: Plasmid]]
-[[Category: Trimethoprim resistance methotrexate resistance]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:34:02 2008''