1hwu: Difference between revisions
New page: left|200px<br /><applet load="1hwu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hwu, resolution 2.1Å" /> '''STRUCTURE OF PII PROT... |
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== | ==STRUCTURE OF PII PROTEIN FROM HERBASPIRILLUM SEROPEDICAE== | ||
PII-like proteins are signal transduction proteins found in bacteria, archaea and eukaryotes. They mediate a variety of cellular responses. A | <StructureSection load='1hwu' size='340' side='right'caption='[[1hwu]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1hwu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Herbaspirillum_seropedicae Herbaspirillum seropedicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwu OCA], [https://pdbe.org/1hwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwu RCSB], [https://www.ebi.ac.uk/pdbsum/1hwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/P94852_HERSE P94852_HERSE] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/1hwu_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hwu ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
PII-like proteins are signal transduction proteins found in bacteria, archaea and eukaryotes. They mediate a variety of cellular responses. A second PII-like protein, called GlnK, has been found in several organisms. In the diazotroph Herbaspirillum seropedicae, PII protein is involved in sensing nitrogen levels and controlling nitrogen fixation genes. In this work, the crystal structure of the unliganded H. seropedicae PII was solved by X-ray diffraction. H. seropedicae PII has a Gly residue, Gly108 preceding Pro109 and the main-chain forms a beta turn. The glycine at position 108 allows a bend in the C-terminal main-chain, thereby modifying the surface of the cleft between monomers and potentially changing function. The structure suggests that the C-terminal region of PII proteins may be involved in specificity of function, and nonenteric diazotrophs are found to have the C-terminal consensus XGXDAX(107-112). We are also proposing binding sites for ATP and 2-oxoglutarate based on the structural alignment of PII with PII-ATP/GlnK-ATP, 5-carboxymethyl-2-hydroxymuconate isomerase and 4-oxalocrotonate tautomerase bound to the inhibitor 2-oxo-3-pentynoate. | |||
Herbaspirillum seropedicae signal transduction protein PII is structurally similar to the enteric GlnK.,Machado Benelli E, Buck M, Polikarpov I, Maltempi de Souza E, Cruz LM, Pedrosa FO Eur J Biochem. 2002 Jul;269(13):3296-303. PMID:12084071<ref>PMID:12084071</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1hwu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Herbaspirillum seropedicae]] | [[Category: Herbaspirillum seropedicae]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Benelli | [[Category: Benelli EM]] | ||
[[Category: Buck | [[Category: Buck M]] | ||
[[Category: Cruz | [[Category: Cruz LM]] | ||
[[Category: De Souza EM]] | |||
[[Category: Pedrosa FO]] | |||
[[Category: Souza | [[Category: Polikarpov I]] | ||
[[Category: | |||
[[Category: | |||
